中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2010年
2期
152-154
,共3页
徐玲%李佳%蒋岚%徐勇%钟海花
徐玲%李佳%蔣嵐%徐勇%鐘海花
서령%리가%장람%서용%종해화
糖尿病肾病%高糖%NF-κB%MG132%泛素-蛋白酶体途径
糖尿病腎病%高糖%NF-κB%MG132%汎素-蛋白酶體途徑
당뇨병신병%고당%NF-κB%MG132%범소-단백매체도경
Diabetic nephropathy%High glucose%NF-κB%MG132%Ubiquitin-proteasome pathway
探讨高糖对肾系膜细胞IκB-α mRNA和IKB-α、NF-κB p65蛋白表达和NF-κB p65核转位的影响及作用途径.结果发现高糖促进IκB-α蛋白降解和NF-κB p65核转位,特异性泛素-蛋白酶体阻断剂MG132可明显逆转高糖导致的IκB-α蛋白降解和NF-κB p65核转位,提示高糖可通过泛素-蛋白酶体途径导致NF-κB信号通路的活化.
探討高糖對腎繫膜細胞IκB-α mRNA和IKB-α、NF-κB p65蛋白錶達和NF-κB p65覈轉位的影響及作用途徑.結果髮現高糖促進IκB-α蛋白降解和NF-κB p65覈轉位,特異性汎素-蛋白酶體阻斷劑MG132可明顯逆轉高糖導緻的IκB-α蛋白降解和NF-κB p65覈轉位,提示高糖可通過汎素-蛋白酶體途徑導緻NF-κB信號通路的活化.
탐토고당대신계막세포IκB-α mRNA화IKB-α、NF-κB p65단백표체화NF-κB p65핵전위적영향급작용도경.결과발현고당촉진IκB-α단백강해화NF-κB p65핵전위,특이성범소-단백매체조단제MG132가명현역전고당도치적IκB-α단백강해화NF-κB p65핵전위,제시고당가통과범소-단백매체도경도치NF-κB신호통로적활화.
To explore the effect of high glucose on the NF-κB/IκB signal pathway in cultured rat glomerular mesangial cells. The results showed that high glucose increased the degradation of IκB-α and the translocation to nucleus of NF-κB. These changes could be reverted mostly by MG132, a proteasome inhibitor. It suggests that the activation of the NF-κB signal pathway by high glucose concentration may probably be via the ubiquitin-proteasome pathway.
