中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
10期
689-692
,共4页
膀胱炎%间质性%T淋巴细胞%免疫活性
膀胱炎%間質性%T淋巴細胞%免疫活性
방광염%간질성%T림파세포%면역활성
Cystitis,interstitial%T-lymphocytes%Immunocompetence
目的 探讨间质性膀胱炎(IC)患者血浆及膀胱黏膜1型/2型辅助性T淋巴细胞(Th1/Th2)与免疫调节失衡的关系.方法 女性IC患者血浆及黏膜标本16例,输尿管结石患者标本16例作为对照组.2组患者性别及年龄匹配.IC患者符合美国糖尿病、消化及肾病协会诊断标准.依据盆腔疼痛和尿急/尿频症状评分(PUF评分)结果分为低评分(0～20分)和高评分(21～35分)2组.采用酶联免疫吸附试验和免疫组化染色检测2组患者血浆和膀胱黏膜中Th1(IFN-γ和IL2)和Th2(IL-4和IL-10)细胞因子表达情况. 结果①IC患者血浆IFN-γ、IL-2表达量分别为(4.57±2.92)、(17.52±7.52)pg/ml,对照组分别为(4.11±2.27)、(20.99±5.09)pg/ml,2组间比较差异均无统计学意义(P＞0.05);IC患者血浆IL-4、IL-10表达量分别为(O.34±0.22)、(4.37±2.34)pg/ml,对照组分别为(0.14±0.07)、(1.18±0.61)pg/ml,2组间比较差异均有统计学意义(P＜0.05).②IC患者和对照组膀胱黏膜标本中IFN-γ、IL-2表达强度比较χ2值=1.900、0.514,差异均无统计学意义(P＞0.05);2组IL-4、IL-10表达强度比较χ2值=8.237、8.209,差异均有统计学意义(P＜0.05).③IC患者PUF低评分组血浆IL-4、IL-10表达量分别为(0.16±0.11)、(2.42±1.27)pg/ml,高评分组分别为(0.53±0.12)、(6.31±1.21)pg/ml,2组间比较差异均有统计学意义(P＜0.05).2组膀胱黏膜标本IL-4表达强度比较χ2＝7.608,γ3＝0.467,差异有统计学意义(P＜0.05);IL-10表达强度比较χ2＝8.844,γ3＝0.517,差异有统计学意义(P＜0.05).结论 IC患者存在免疫调节失衡,Th2介导的体液免疫反应增强,且免疫失衡随症状程度而加重.
目的 探討間質性膀胱炎(IC)患者血漿及膀胱黏膜1型/2型輔助性T淋巴細胞(Th1/Th2)與免疫調節失衡的關繫.方法 女性IC患者血漿及黏膜標本16例,輸尿管結石患者標本16例作為對照組.2組患者性彆及年齡匹配.IC患者符閤美國糖尿病、消化及腎病協會診斷標準.依據盆腔疼痛和尿急/尿頻癥狀評分(PUF評分)結果分為低評分(0～20分)和高評分(21～35分)2組.採用酶聯免疫吸附試驗和免疫組化染色檢測2組患者血漿和膀胱黏膜中Th1(IFN-γ和IL2)和Th2(IL-4和IL-10)細胞因子錶達情況. 結果①IC患者血漿IFN-γ、IL-2錶達量分彆為(4.57±2.92)、(17.52±7.52)pg/ml,對照組分彆為(4.11±2.27)、(20.99±5.09)pg/ml,2組間比較差異均無統計學意義(P＞0.05);IC患者血漿IL-4、IL-10錶達量分彆為(O.34±0.22)、(4.37±2.34)pg/ml,對照組分彆為(0.14±0.07)、(1.18±0.61)pg/ml,2組間比較差異均有統計學意義(P＜0.05).②IC患者和對照組膀胱黏膜標本中IFN-γ、IL-2錶達彊度比較χ2值=1.900、0.514,差異均無統計學意義(P＞0.05);2組IL-4、IL-10錶達彊度比較χ2值=8.237、8.209,差異均有統計學意義(P＜0.05).③IC患者PUF低評分組血漿IL-4、IL-10錶達量分彆為(0.16±0.11)、(2.42±1.27)pg/ml,高評分組分彆為(0.53±0.12)、(6.31±1.21)pg/ml,2組間比較差異均有統計學意義(P＜0.05).2組膀胱黏膜標本IL-4錶達彊度比較χ2＝7.608,γ3＝0.467,差異有統計學意義(P＜0.05);IL-10錶達彊度比較χ2＝8.844,γ3＝0.517,差異有統計學意義(P＜0.05).結論 IC患者存在免疫調節失衡,Th2介導的體液免疫反應增彊,且免疫失衡隨癥狀程度而加重.
목적 탐토간질성방광염(IC)환자혈장급방광점막1형/2형보조성T림파세포(Th1/Th2)여면역조절실형적관계.방법 녀성IC환자혈장급점막표본16례,수뇨관결석환자표본16례작위대조조.2조환자성별급년령필배.IC환자부합미국당뇨병、소화급신병협회진단표준.의거분강동통화뇨급/뇨빈증상평분(PUF평분)결과분위저평분(0～20분)화고평분(21～35분)2조.채용매련면역흡부시험화면역조화염색검측2조환자혈장화방광점막중Th1(IFN-γ화IL2)화Th2(IL-4화IL-10)세포인자표체정황. 결과①IC환자혈장IFN-γ、IL-2표체량분별위(4.57±2.92)、(17.52±7.52)pg/ml,대조조분별위(4.11±2.27)、(20.99±5.09)pg/ml,2조간비교차이균무통계학의의(P＞0.05);IC환자혈장IL-4、IL-10표체량분별위(O.34±0.22)、(4.37±2.34)pg/ml,대조조분별위(0.14±0.07)、(1.18±0.61)pg/ml,2조간비교차이균유통계학의의(P＜0.05).②IC환자화대조조방광점막표본중IFN-γ、IL-2표체강도비교χ2치=1.900、0.514,차이균무통계학의의(P＞0.05);2조IL-4、IL-10표체강도비교χ2치=8.237、8.209,차이균유통계학의의(P＜0.05).③IC환자PUF저평분조혈장IL-4、IL-10표체량분별위(0.16±0.11)、(2.42±1.27)pg/ml,고평분조분별위(0.53±0.12)、(6.31±1.21)pg/ml,2조간비교차이균유통계학의의(P＜0.05).2조방광점막표본IL-4표체강도비교χ2＝7.608,γ3＝0.467,차이유통계학의의(P＜0.05);IL-10표체강도비교χ2＝8.844,γ3＝0.517,차이유통계학의의(P＜0.05).결론 IC환자존재면역조절실형,Th2개도적체액면역반응증강,차면역실형수증상정도이가중.
Objective To investigate possible disturbance of Th1/Th2 immunoregulation of blood serum and bladder mucosa in patients with interstitial cystitis(IC). Methods Blood serum and bladder mucosal specimens were collected from 16 female patients with IC and 16 female normal controis.The age of IC patients was 51.3±10.2 years.The age of normal controls was 53.1±9.6years.The expressions of Th1(IFN-γ,IL-2)and Th2 cytokines(IL-4,IL-10)were determined by enIFN-γ and IL-2 in IC blood serum were(4.57±2.92),(17.52±7.52)pg/ml and in normal blood serum were(4.11±2.27),(20.99±5.09)pg/ml.Such two factors had no significant differences in these two groups.The expressions of IL-4 and IL-10 in IC blood serum were(0.34±0.22),(4.37±2.34)pg/ml and in normal blood serum were(O.14±O.07),(1.18±0.61)pg/ml.Such two factors had sighad no significant differences with normal bladder tissues(IFN-γ:χ2＝1.900,IL-2:χ2＝0.514).The expressions of IL-4 and IL-10 in IC bladder tissues had significant differences with normal bladder tiswere(0.16±0.11)and(2.42±1.27)pg/ml but in high PUF group were(0.53±0.12)and(6.31±1.21)pg/ml.The expressions of these two factors had significant correlations with the degree of PUF,which intensified in the IC of high PUF. Conclusion The expression of Th2 type eytokines is predominant in IC blood plasm and bladder tissues,the expression of Th2 type cytokines in IC blood plasm and bladder tissues has positive correlation with the degree of PUF.
