国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
9期
791-794
,共4页
周海哲%李军%赵欢%曹永升
週海哲%李軍%趙歡%曹永升
주해철%리군%조환%조영승
缺血性中风%大鼠%脑泰通颗粒
缺血性中風%大鼠%腦泰通顆粒
결혈성중풍%대서%뇌태통과립
Ischemic apoplexy%Rat%Naotaitong granule
目的 观察脑泰通颗粒对缺血性脑卒中模型的作用，初步探究其治疗缺血性脑卒中的可能机制。方法 对128只大鼠进行高脂血症造模，然后随机抽出30只作为假手术组，其余动物进行缺血性脑卒中造模，术后按随机数字表法随机分为安慰剂组、脑泰通颗粒组及阳性对照组，于造模后第2天开始灌胃给药，假手术组、安慰剂组给予生理盐水1 ml/100 g·d-1;脑泰通颗粒组给予脑泰通颗粒水溶液1 ml/100 g·d-1，阳性对照组给予吡拉西坦片0.6 g/100 g·d-1。各组均1次/d，持续4周，并分别于用药后7d、10d、14d、21d、28 d观察大鼠的行为学改变、HE染色大鼠脑组织神经元变化及免疫组化方法检测脑组织中神经生长导向因子Slit2和血管内皮生长因子(VEGF)的表达情况。结果 造模后，动物均表现出病理征、偏瘫等局灶性神经症状，脑组织亦有明显软化灶。用药后实验组及阳性对照组动物改善良好，软化灶明显缩小。安慰剂组在7d、10d、14 d、21d、28 d的Longa评分分别为(2.92±0.20)分、(2.58±0.20)分、(2.25±0.27)分、(1.83±0.26)分、(1.42±0.20)分；脑泰通颗粒组评分分别为(1.92±0.20)分、(1.50±0.32)分、(1.25±0.27)分、(0.83±0.26)分、(0.50±0.00)分；阳性对照组评分分别为(2.33±0.41)分、(2.00±0.45)分、(1.75±0.27)分、(1.33±0.41)分、(0.92±0.38)分。与安慰剂组比较均降低（P均＜0.01）。结论 脑泰通颗粒可改善大鼠脑组织缺血缺氧状态，具有保护脑组织作用。
目的 觀察腦泰通顆粒對缺血性腦卒中模型的作用，初步探究其治療缺血性腦卒中的可能機製。方法 對128隻大鼠進行高脂血癥造模，然後隨機抽齣30隻作為假手術組，其餘動物進行缺血性腦卒中造模，術後按隨機數字錶法隨機分為安慰劑組、腦泰通顆粒組及暘性對照組，于造模後第2天開始灌胃給藥，假手術組、安慰劑組給予生理鹽水1 ml/100 g·d-1;腦泰通顆粒組給予腦泰通顆粒水溶液1 ml/100 g·d-1，暘性對照組給予吡拉西坦片0.6 g/100 g·d-1。各組均1次/d，持續4週，併分彆于用藥後7d、10d、14d、21d、28 d觀察大鼠的行為學改變、HE染色大鼠腦組織神經元變化及免疫組化方法檢測腦組織中神經生長導嚮因子Slit2和血管內皮生長因子(VEGF)的錶達情況。結果 造模後，動物均錶現齣病理徵、偏癱等跼竈性神經癥狀，腦組織亦有明顯軟化竈。用藥後實驗組及暘性對照組動物改善良好，軟化竈明顯縮小。安慰劑組在7d、10d、14 d、21d、28 d的Longa評分分彆為(2.92±0.20)分、(2.58±0.20)分、(2.25±0.27)分、(1.83±0.26)分、(1.42±0.20)分；腦泰通顆粒組評分分彆為(1.92±0.20)分、(1.50±0.32)分、(1.25±0.27)分、(0.83±0.26)分、(0.50±0.00)分；暘性對照組評分分彆為(2.33±0.41)分、(2.00±0.45)分、(1.75±0.27)分、(1.33±0.41)分、(0.92±0.38)分。與安慰劑組比較均降低（P均＜0.01）。結論 腦泰通顆粒可改善大鼠腦組織缺血缺氧狀態，具有保護腦組織作用。
목적 관찰뇌태통과립대결혈성뇌졸중모형적작용，초보탐구기치료결혈성뇌졸중적가능궤제。방법 대128지대서진행고지혈증조모，연후수궤추출30지작위가수술조，기여동물진행결혈성뇌졸중조모，술후안수궤수자표법수궤분위안위제조、뇌태통과립조급양성대조조，우조모후제2천개시관위급약，가수술조、안위제조급여생리염수1 ml/100 g·d-1;뇌태통과립조급여뇌태통과립수용액1 ml/100 g·d-1，양성대조조급여필랍서탄편0.6 g/100 g·d-1。각조균1차/d，지속4주，병분별우용약후7d、10d、14d、21d、28 d관찰대서적행위학개변、HE염색대서뇌조직신경원변화급면역조화방법검측뇌조직중신경생장도향인자Slit2화혈관내피생장인자(VEGF)적표체정황。결과 조모후，동물균표현출병리정、편탄등국조성신경증상，뇌조직역유명현연화조。용약후실험조급양성대조조동물개선량호，연화조명현축소。안위제조재7d、10d、14 d、21d、28 d적Longa평분분별위(2.92±0.20)분、(2.58±0.20)분、(2.25±0.27)분、(1.83±0.26)분、(1.42±0.20)분；뇌태통과립조평분분별위(1.92±0.20)분、(1.50±0.32)분、(1.25±0.27)분、(0.83±0.26)분、(0.50±0.00)분；양성대조조평분분별위(2.33±0.41)분、(2.00±0.45)분、(1.75±0.27)분、(1.33±0.41)분、(0.92±0.38)분。여안위제조비교균강저（P균＜0.01）。결론 뇌태통과립가개선대서뇌조직결혈결양상태，구유보호뇌조직작용。
objective To explore the mechanism of Naotaitong granule on ischemic apoplexy by observing the effect of Naotaitong granule on ischemic apoplexy model. Methods 128 rats were randomly divided into sham operation group, console group, experimental group and control group. Ischemic apoplexy model were established in all the animals. Different disposal was given to different group. In the 7 d、 10 d、 14 d、21 d and 28 d after drug administration, immunohistochemistry were used to evaluate the expression of Slit2 and VEGF. In addition, behavior alteration and HE staining were applied to evaluate the variation of brain tissue neuron. Results All the animals revealed hemiplegia and cerebral tissue softened after successfully establishing apoplexy model. The morphology of experimental group and control group was improved after drug administration with softened focus obviously reduced. Longa grade of console group in the 7 d、 10 d、 14 d、 21 d and 28 d was (2.92±0.20)、 (2.58±0.20)、 (2.25±0.27)、 (1.83±0.26) and (1.42±0.20) respectively. Longa grade of Naotaitong group in the 7 d、 10 d、 14 d、 21 d and 28 d was (1.92±0.20)、 (1.50±0.32)、 (1.25±0.27)、(0.83 ±0.26) and (0.50±0.00) respectively. Longa grade of control group in the 7 d、 10 d、 14 d、 21 d and 28 d was (2.33±0.41)、 (2.00±0.45)、 (1.75±0.27)、 (1.33±0.41) and (0.92±0.38) respectively. Longa grade of both control and Naotaitong group had statistics significance compared with the console group (P＜0.01) .Conclusion Naotaitong granule may improve anoxemia in rats brain and protect brain tissue.
