中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2009年
1期
50-51
,共2页
癌,肝细胞%CO2%气腹%HIF-1%VEGF
癌,肝細胞%CO2%氣腹%HIF-1%VEGF
암,간세포%CO2%기복%HIF-1%VEGF
Carcinoma,hepatocellular%CO2%Pneumoperitoneum%HIF-1%VEGF
目的 观察不同CO2气腹压力条件下,大鼠移植性肝癌中缺氧诱导因子(HIF)-1α与血管内皮生长因子(VEGF)表达的变化.方法 制作大鼠Walker-256移植性肝癌模型,将48只肝痛模型随机分为4组,给予不同气腹压力0、5、10、15 mm Hg(1 mm Hg=0.133 kPa),恒定一定时间及流量.术后处死全部大鼠,切取肝肿瘤,4%甲醛固定后行苏木素-伊红(HE)染色,免疫组织化学染色检测HIF-1α及VEGF表达.结果 随着CO2气腹压力的增加,HIF-1α、VEGF在肿瘤模型上的表达逐渐增强(P＜0.05),各组肝癌组织中HIF-1α VEGF呈正相关关系(其r分别为0.835、0.714、0.761、0.781).结论 随着CO2气腹压力的增加,HIF-1α与VEGF的表达逐渐增加,且HIF-1α与VEGF呈正相关.CO2气腹促进肿瘤生长和转移可能与HIF-1α VEGF有一定关系.
目的 觀察不同CO2氣腹壓力條件下,大鼠移植性肝癌中缺氧誘導因子(HIF)-1α與血管內皮生長因子(VEGF)錶達的變化.方法 製作大鼠Walker-256移植性肝癌模型,將48隻肝痛模型隨機分為4組,給予不同氣腹壓力0、5、10、15 mm Hg(1 mm Hg=0.133 kPa),恆定一定時間及流量.術後處死全部大鼠,切取肝腫瘤,4%甲醛固定後行囌木素-伊紅(HE)染色,免疫組織化學染色檢測HIF-1α及VEGF錶達.結果 隨著CO2氣腹壓力的增加,HIF-1α、VEGF在腫瘤模型上的錶達逐漸增彊(P＜0.05),各組肝癌組織中HIF-1α VEGF呈正相關關繫(其r分彆為0.835、0.714、0.761、0.781).結論 隨著CO2氣腹壓力的增加,HIF-1α與VEGF的錶達逐漸增加,且HIF-1α與VEGF呈正相關.CO2氣腹促進腫瘤生長和轉移可能與HIF-1α VEGF有一定關繫.
목적 관찰불동CO2기복압력조건하,대서이식성간암중결양유도인자(HIF)-1α여혈관내피생장인자(VEGF)표체적변화.방법 제작대서Walker-256이식성간암모형,장48지간통모형수궤분위4조,급여불동기복압력0、5、10、15 mm Hg(1 mm Hg=0.133 kPa),항정일정시간급류량.술후처사전부대서,절취간종류,4%갑철고정후행소목소-이홍(HE)염색,면역조직화학염색검측HIF-1α급VEGF표체.결과 수착CO2기복압력적증가,HIF-1α、VEGF재종류모형상적표체축점증강(P＜0.05),각조간암조직중HIF-1α VEGF정정상관관계(기r분별위0.835、0.714、0.761、0.781).결론 수착CO2기복압력적증가,HIF-1α여VEGF적표체축점증가,차HIF-1α여VEGF정정상관.CO2기복촉진종류생장화전이가능여HIF-1α VEGF유일정관계.
Objective To observes the influence of CO2 pneumoperitoneum with different pressure oil the expression of hypoxia-inducible factor 1α(HIF-1α)and vascular endothelial growth factor (VEGF)in transplanted liver cancer model of rats.Methods Transplanted liver cancer model of rats with Walker-256 was established.Forty-eight liver cancer models were randomly divided into 4 groups of 0,5,10,and 15 mmHg pneumoperitoneum pressure(The time and flow rate were permanent).Au rats after oDeration were killed,and the liver tumors were removed,fixed with 4%formaldehyde.and stained with HE.By using immunohistochemistry staining.the expression of HIF-1α and VEGF was detected.Results With the increased of CO2 pneumoperitoneum pressure,the expression of HIF-1α and VEGF was increased gradually on the tumor model(P＜0.05).There was a positive correlation between HIF-1α and VEGF in liver cancer tissues of each group(r=0.835,0.714,0.761,0.781.respectively).Conclusion Promotion of tumor growth and metastasis by CO2 pneumoperitoneum has certain relation with HIF-1α and VEGF.There is a positive correlation between HIF-1α and VEGF.
