中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2009年
2期
122-126
,共5页
骨髓间充质干细胞%藻酸盐%软骨分化%软骨组织工程
骨髓間充質榦細胞%藻痠鹽%軟骨分化%軟骨組織工程
골수간충질간세포%조산염%연골분화%연골조직공정
Bone marrow mesenehymal stem cell%Alginate%Chondrogenesis%Cartilage tissue engineering
目的 探讨β3转化生长因子(TGF-β3)在诱导骨髓间充质干细胞(mesenchymal stem cells,MSCs)向软骨细胞分化中胰岛素样生长因子-1(IGF-1)的作用以及在软骨组织工程中的应用. 方法 在体外用TGF-β3或(和)IGF-1诱导藻酸钠微球巾的MSCs向软骨细胞定向分化,免疫组织化学、逆转录-聚合酶链反应(RT-PCR)检测Ⅱ型胶原和聚集蛋白聚糖(aggrecan)的表达,Western印迹法检测Sox9蛋白的表达,激光共聚焦显微镜和扫描电镜观察该软骨细胞在壳聚糖支架上的生长.结果 TGF-β3能诱导藻酸钠微球中的MSCs表达软骨特异性的Ⅱ型胶原、聚集蛋白聚糖和Sox9,IGF-1能显著性地增强这种作用(P＜0.05).Ⅱ型胶原、聚集蛋白聚糖和Sox9之间的相关系数分别为0.95和0.91.诱导的软骨细胞能在壳聚糖支架上黏附、迁徙、增殖.结论在TGF-β3诱导MSCs分化成软骨细胞地过程中,IGF-1可能通过促进Sox9的表达起到协同作用.诱导分化后的软骨细胞与壳聚糖复合支架表现出良好的组织相容性.
目的 探討β3轉化生長因子(TGF-β3)在誘導骨髓間充質榦細胞(mesenchymal stem cells,MSCs)嚮軟骨細胞分化中胰島素樣生長因子-1(IGF-1)的作用以及在軟骨組織工程中的應用. 方法 在體外用TGF-β3或(和)IGF-1誘導藻痠鈉微毬巾的MSCs嚮軟骨細胞定嚮分化,免疫組織化學、逆轉錄-聚閤酶鏈反應(RT-PCR)檢測Ⅱ型膠原和聚集蛋白聚糖(aggrecan)的錶達,Western印跡法檢測Sox9蛋白的錶達,激光共聚焦顯微鏡和掃描電鏡觀察該軟骨細胞在殼聚糖支架上的生長.結果 TGF-β3能誘導藻痠鈉微毬中的MSCs錶達軟骨特異性的Ⅱ型膠原、聚集蛋白聚糖和Sox9,IGF-1能顯著性地增彊這種作用(P＜0.05).Ⅱ型膠原、聚集蛋白聚糖和Sox9之間的相關繫數分彆為0.95和0.91.誘導的軟骨細胞能在殼聚糖支架上黏附、遷徙、增殖.結論在TGF-β3誘導MSCs分化成軟骨細胞地過程中,IGF-1可能通過促進Sox9的錶達起到協同作用.誘導分化後的軟骨細胞與殼聚糖複閤支架錶現齣良好的組織相容性.
목적 탐토β3전화생장인자(TGF-β3)재유도골수간충질간세포(mesenchymal stem cells,MSCs)향연골세포분화중이도소양생장인자-1(IGF-1)적작용이급재연골조직공정중적응용. 방법 재체외용TGF-β3혹(화)IGF-1유도조산납미구건적MSCs향연골세포정향분화,면역조직화학、역전록-취합매련반응(RT-PCR)검측Ⅱ형효원화취집단백취당(aggrecan)적표체,Western인적법검측Sox9단백적표체,격광공취초현미경화소묘전경관찰해연골세포재각취당지가상적생장.결과 TGF-β3능유도조산납미구중적MSCs표체연골특이성적Ⅱ형효원、취집단백취당화Sox9,IGF-1능현저성지증강저충작용(P＜0.05).Ⅱ형효원、취집단백취당화Sox9지간적상관계수분별위0.95화0.91.유도적연골세포능재각취당지가상점부、천사、증식.결론재TGF-β3유도MSCs분화성연골세포지과정중,IGF-1가능통과촉진Sox9적표체기도협동작용.유도분화후적연골세포여각취당복합지가표현출량호적조직상용성.
Objective To investigate the effect of IGF-1 on the TGF-β3 induced chondrogenesis of MSCs encapsulated in alginate beads and its application in cartilage tissue engineering.Methods MSC chondrogenesis in alginate beads was induced by TGF-β3 and/or IGF-1.Collagen type Ⅱ,aggrecan and Sox-9 expression was evaluated by immunostaining,RT-PCR and Western blot,respectively.Scanning electron microscope and laser confocal microscope were used to observe the differentiated chondrocytes when cultured on the chitosan-based scaffold.Results TGF-β3 with IGF-1 induced MSCs in alginate beads to express the higher level of collagen type Ⅱ,aggrecan and Sox-9 than any other growth factor alone (P＜0.05).The correlation coefficient between Sox9 and collagen type Ⅱ or aggrecan was 0.95 and 0.91,respectively.The chitosan-based scaffold supported the cell's adhesion,migration and proliferation.Conlusion IGF-1 enhances the TGF-β3-induced MSC chondrogenesis via upregulating Sox9 expression.The chitosan-based scaffold is biocompatible with the differentiated chondrocytes.
