CAJ | 학술논문

目的:构建并鉴定真核表达质粒pcDNA3.1(+)-MCP-1和pcDNA3.1(+)-Groα.方法:据GeneBank中大鼠MCP-1和GroαcDNA序列设计并合成引物,提取急性胰腺炎模型大鼠总RNA,RT-PCR扩增,并将扩增产物TA克隆至pGEM-T easy载体,然后分别双酶切pGEM-MCP-1和pGEM-Groα回收目的片段再克隆至真核表达载体pcDNA3.1(+).结果:pcDNA3.1(+)-MCP-1和pcDNA3.1(+)-Groα真核表达质粒构建完成后,用限制性内切酶、PCR及DNA序列分析等多种方法进行鉴定,证实其构建成功.结论:pcDNA3.1(+)-MCP-1和pcDNA3.1(+)-Groα真核表达质粒构建成功,为进一步研究该真核表达质粒的免疫保护效果及制备急性胰腺炎pcDNA3.1(+)-MCP-1和pcDNA3.1(+)-Gro DNA疫苗奠定了基础.
목적:구건병감정진핵표체질립pcDNA3.1(+)-MCP-1화pcDNA3.1(+)-Groα.방법:거GeneBank중대서MCP-1화GroαcDNA서렬설계병합성인물,제취급성이선염모형대서총RNA,RT-PCR확증,병장확증산물TA극륭지pGEM-T easy재체,연후분별쌍매절pGEM-MCP-1화pGEM-Groα회수목적편단재극륭지진핵표체재체pcDNA3.1(+).결과:pcDNA3.1(+)-MCP-1화pcDNA3.1(+)-Groα진핵표체질립구건완성후,용한제성내절매、PCR급DNA서렬분석등다충방법진행감정,증실기구건성공.결론:pcDNA3.1(+)-MCP-1화pcDNA3.1(+)-Groα진핵표체질립구건성공,위진일보연구해진핵표체질립적면역보호효과급제비급성이선염pcDNA3.1(+)-MCP-1화pcDNA3.1(+)-Gro DNA역묘전정료기출.