CAJ | 학술논문

采用生物信息学的方法和工具对已在GenBaak上注册的黑腹果蝇（Drosophila melanogaster）、致倦库蚊（Culexpi piensquin quefasciatus）、埃及伊蚊（Aedes aegypti）、意大利蜜蜂（Apis mellifera）、丽蝇蛹集金小蜂（Nasonia vitripennis）的雌激素相关受体ERR的核酸及氨基酸序列进行分析，并对其组成成分、理化性质、疏水性／亲水性、蛋白质二级及三级结构、分子系统进化关系等进行了预测和推断．结果表明：昆虫ERR是一个亲水性蛋白．在ERR整体蛋白结构中α-螺旋和不规则卷曲是ERR最大量的结构元件，而β-折叠则散布于整个蛋白质中．ERR蛋白的DNA结合区（DID）主要由三个α-螺旋和三个β-折叠构成，包含两个锌指结构．配体结合区（LBD）主要由3个β-折叠和11个α-螺旋构成．
채용생물신식학적방법화공구대이재GenBaak상주책적흑복과승（Drosophila melanogaster）、치권고문（Culexpi piensquin quefasciatus）、애급이문（Aedes aegypti）、의대리밀봉（Apis mellifera）、려승용집금소봉（Nasonia vitripennis）적자격소상관수체ERR적핵산급안기산서렬진행분석，병대기조성성분、이화성질、소수성／친수성、단백질이급급삼급결구、분자계통진화관계등진행료예측화추단．결과표명：곤충ERR시일개친수성단백．재ERR정체단백결구중α-라선화불규칙권곡시ERR최대량적결구원건，이β-절첩칙산포우정개단백질중．ERR단백적DNA결합구（DID）주요유삼개α-라선화삼개β-절첩구성，포함량개자지결구．배체결합구（LBD）주요유3개β-절첩화11개α-라선구성．
In the present study, estrogen- related receptors （ERR）from Drosophila melanogaster, Culex pipierm quinquefasciatus, Aedes aegypti, Nasonia vitripennis and Apis mellifera which were registered in Genbank, were analyzed and predicted by the tools of bioinformatics in the following aspects： the composition of nuclei acid sequences and amino acid sequences, protein physical and chemical properties, hydrophobicity or hydrophilicity, secondary and tertiary structures of protein, molecular phylogenetic evolution and so on. The results are as follows： ERR is a hydrophilic protein; the main motif of predicted secondary structure of ERR are alpha helix and random coil, beta turn and extended strand are spread in the whole secondary structure of protein;there are three β-strands and three α-helixs in DNA binding domain （DBD）, and three β - strands and eleven α-helixs in ligand binding domain （LBD）. The DBD is characterised by two Zinc-finger structures.