CAJ | 학술논문

采用电喷雾质谱和串联质谱以及聚丙烯酰胺凝胶电泳技术研究了[CuL(H2O)](BF4)2(L为2-[二(2-氨乙酸)氨基]乙醇)与马心肌红蛋白的键合作用和水解切割.聚丙烯酰胺凝胶电泳研究显示在中性及60℃条件下,切割效率与[CuL(H2O)]2+的浓度和温育时间密切相关.电喷雾质谱和串联质谱分析显示,[CuL(H2O)]2+通过与肌红蛋白的氨基酸His36,His93,His116和Arg139侧链的结合,并在羟乙基侧臂的促进下,选择性地水解了肽键Phe33-Thr34,Gln91-Ser92,Ala94-Thr95,His116-Ser117和Asn140-Asp141.
채용전분무질보화천련질보이급취병희선알응효전영기술연구료[CuL(H2O)](BF4)2(L위2-[이(2-안을산)안기]을순)여마심기홍단백적건합작용화수해절할.취병희선알응효전영연구현시재중성급60℃조건하,절할효솔여[CuL(H2O)]2+적농도화온육시간밀절상관.전분무질보화천련질보분석현시,[CuL(H2O)]2+통과여기홍단백적안기산His36,His93,His116화Arg139측련적결합,병재간을기측비적촉진하,선택성지수해료태건Phe33-Thr34,Gln91-Ser92,Ala94-Thr95,His116-Ser117화Asn140-Asp141.
The bonding interaction and hydrolytic cleavage of horse heart myoglobin with [CuL(H2O)](BF4)2, where L is 2-[bis (2-aminoethyl)amino]ethanol, were investigated by electrospray ionization mass spectrometry(ESI-MS),tandem mass spectrometry (MS/MS) and SDS-PAGE electrophoresis. The SDS-PAGE electrophoresis showed that the cleavage yield was dependent on the concentration of [CuL(H2O)]2+ and incubation time. The ESI-MS and MS/MS analysis revealed that with the assistance of the pendant hydroxyl group in [CuL(H2O)]2+, [CuL(H2O)]2+ may selectively hydrolysis the peptide bonds of Phe33-Thr34, Gln91-Ser92, Ala94-Thr95, His 116-Ser117 and Asn 140-Asp141 of myoglobin by the binding of [CuL(H2O)]2+ to the side chains of His36, His93, His116 and Arg139 of myoglobin.