CAJ | 학술논문

目的探讨E-选择素在结肠癌肝转移中的作用,并筛选有临床应用前景的抗结肠癌转移药物.方法检测E-选择素在肝静脉内皮细胞( ED25)中的表达, 检测活化ED25与结肠癌细胞Love、肝癌细胞HepG2、鼻咽癌细胞在体外的黏附差异,并测试不同药物对黏附的影响.结果 E-选择素高强度表达于活化后的ED25,且能介导Love与ED25的黏附.Love条件培养液处理ED25可增强与Love、HepG2、鼻咽癌细胞的黏附,但Love和HepG2与ED25的黏附明显强于鼻咽癌细胞(P<0.05),而Love和HepG2与ED25的黏附差异无显著性意义(P=0.29);抗E-选择素抗体、地塞米松、丝裂霉素、5-FU有较强抑制结肠癌细胞与ED25黏附作用.结论 E-选择素可促进结肠癌细胞与ED25的黏附;结肠癌细胞可分泌促进ED25表达E-选择素表达的物质, 从而促进了结肠向肝脏癌的转移;地塞米松、丝裂霉素、5-FU可以抑制结肠癌细胞与ED25的黏附, 有望成为预防结肠癌肝转移的药物.
목적 탐토E-선택소재결장암간전이중적작용,병사선유림상응용전경적항결장암전이약물.방법 검측E-선택소재간정맥내피세포( ED25)중적표체, 검측활화ED25여결장암세포Love、간암세포HepG2、비인암세포재체외적점부차이,병측시불동약물대점부적영향.결과 E-선택소고강도표체우활화후적ED25,차능개도Love여ED25적점부.Love조건배양액처리ED25가증강여Love、HepG2、비인암세포적점부,단Love화HepG2여ED25적점부명현강우비인암세포(P<0.05),이Love화HepG2여ED25적점부차이무현저성의의(P=0.29);항E-선택소항체、지새미송、사렬매소、5-FU유교강억제결장암세포여ED25점부작용.결론 E-선택소가촉진결장암세포여ED25적점부;결장암세포가분비촉진ED25표체E-선택소표체적물질, 종이촉진료결장향간장암적전이;지새미송、사렬매소、5-FU가이억제결장암세포여ED25적점부, 유망성위예방결장암간전이적약물.
Objective To explore the role of E-selectin on the liver metastasis of colon carcinoma and select possible drugs to inhibit metastasis of colon carcinoma.Methods Expression of E-selectin in hepatic vein endothelial cell (ED25) was detected.The adherence ability of actived ED25 to colon carcinoma cell (Love),hepatocellular carcinoma cell (HepG2) and nasopharyngeal carcinoma cell were compared in vitro by solid phase adhesion assay.The effect on the tumor cell endothelial adhesion was investigated in different drugs.Results E-selectin was highly expressed on the surface of actived ED25 and could mediate the adhesion of ED25 to Love.The adherence ability was enhanced after ED25 stimulated by Love condition medium in different tumor cells.The adherence ability of actived ED25 to Love and HepG2 were significant higher than that of nasopharyngeal carcinoma cell (P<0.05),while there had no difference in the adherence ability of actived ED25 to Love and HepG2 (P=0.29).Anti-E-selectin antibody,dexamethasone,mitomycin and 5-FU were able to block the adhesion of Love to ED25.Conclusion E-selectin is an important molecular in the adhesion of Love to ED25,and colon carcinoma cell can secrete some factors to promote the expression of E-selectin. Dexamethasone, mitomycin and 5-FU could inhibit the adhesion of Love to ED25, and could be hopeful drugs in inhibiting the metastasis of colon carcinoma.