上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2009年
12期
1463-1466
,共4页
赵树田%张士青%顾欣%李建涛
趙樹田%張士青%顧訢%李建濤
조수전%장사청%고흔%리건도
草酸%降解%乳酸菌%酸奶
草痠%降解%乳痠菌%痠奶
초산%강해%유산균%산내
oxalate%degradation%lactic acid bacteria%yoghourt
目的 评价10种可制作酸奶的乳酸菌在草酸培养液中降解草酸的能力.方法 在5 mmol/L草酸培养液中,分别接种嗜酸乳杆菌、副干酪乳杆菌、屎肠球菌、乳双歧杆菌、青春双歧杆菌、婴儿双歧杆菌、长双歧杆菌、乳酸乳球菌乳脂亚种、保加利亚乳杆菌和嗜热链球菌共10种乳酸菌,培养72 h后检测培养液中草酸浓度和乳酸菌浓度的变化;另设不接种乳酸菌培养的空白对照.结果 经过72 h培养,10种乳酸菌浓度均增加,培养前后差异均有统计学意义(P<0.01).与空白对照相比,所有接种乳酸菌的培养液草酸浓度均下降,其中嗜酸乳杆菌、乳酸乳球菌乳脂亚种、长双歧杆菌、青春双歧杆菌和乳双歧杆菌培养前后的草酸浓度差异具有统计学意义(P<0.05或P<0.01);乳双歧杆菌的草酸降解率(29.03%)最高,屎链球菌草酸降解率(0.23%)最低.相关性分析显示,乳酸菌的增殖倍数与草酸降解率无显著相关性(r=0.435 7, P=0.208 2).结论 10种可制作酸奶的乳酸菌均具有草酸降解能力,草酸降解能力与其在草酸培养液中的增殖能力无相关性.
目的 評價10種可製作痠奶的乳痠菌在草痠培養液中降解草痠的能力.方法 在5 mmol/L草痠培養液中,分彆接種嗜痠乳桿菌、副榦酪乳桿菌、屎腸毬菌、乳雙歧桿菌、青春雙歧桿菌、嬰兒雙歧桿菌、長雙歧桿菌、乳痠乳毬菌乳脂亞種、保加利亞乳桿菌和嗜熱鏈毬菌共10種乳痠菌,培養72 h後檢測培養液中草痠濃度和乳痠菌濃度的變化;另設不接種乳痠菌培養的空白對照.結果 經過72 h培養,10種乳痠菌濃度均增加,培養前後差異均有統計學意義(P<0.01).與空白對照相比,所有接種乳痠菌的培養液草痠濃度均下降,其中嗜痠乳桿菌、乳痠乳毬菌乳脂亞種、長雙歧桿菌、青春雙歧桿菌和乳雙歧桿菌培養前後的草痠濃度差異具有統計學意義(P<0.05或P<0.01);乳雙歧桿菌的草痠降解率(29.03%)最高,屎鏈毬菌草痠降解率(0.23%)最低.相關性分析顯示,乳痠菌的增殖倍數與草痠降解率無顯著相關性(r=0.435 7, P=0.208 2).結論 10種可製作痠奶的乳痠菌均具有草痠降解能力,草痠降解能力與其在草痠培養液中的增殖能力無相關性.
목적 평개10충가제작산내적유산균재초산배양액중강해초산적능력.방법 재5 mmol/L초산배양액중,분별접충기산유간균、부간락유간균、시장구균、유쌍기간균、청춘쌍기간균、영인쌍기간균、장쌍기간균、유산유구균유지아충、보가리아유간균화기열련구균공10충유산균,배양72 h후검측배양액중초산농도화유산균농도적변화;령설불접충유산균배양적공백대조.결과 경과72 h배양,10충유산균농도균증가,배양전후차이균유통계학의의(P<0.01).여공백대조상비,소유접충유산균적배양액초산농도균하강,기중기산유간균、유산유구균유지아충、장쌍기간균、청춘쌍기간균화유쌍기간균배양전후적초산농도차이구유통계학의의(P<0.05혹P<0.01);유쌍기간균적초산강해솔(29.03%)최고,시련구균초산강해솔(0.23%)최저.상관성분석현시,유산균적증식배수여초산강해솔무현저상관성(r=0.435 7, P=0.208 2).결론 10충가제작산내적유산균균구유초산강해능력,초산강해능력여기재초산배양액중적증식능력무상관성.
Objective To investigate the oxalate-degrading abilities of 10 strains of lactic acid bacteria for yoghourt fermentation. Methods Ten different strains of lactic acid bacteria (L.acidophilus, L.paracasei, Enterococcaceae faecium, B. lactis, B. adolescentis, B. infantis, B.longum, Lactococcus lactis subsp. Cremori, L.bulgaricus and S. thermophilus) were cultured separately in culture fluid containing 5 mmol/L oxalate. Seventy-two hours after culture, the concentrations of oxalate and lactic acid bacteria were detected. Besides, blank control cultured without lactic acid bacteria was established. Results Seventy-two hours after culture, the concentrations of all the 10 strains of lactic acid bacteria were significantly higher than those before culture (P<0.01). Compared with blank control, the concentrations of oxalate in the culture fluid with 10 strains of lactic acid bacteria decreased 72 h after culture, and were significantly different from those before culture for L.acidophilus, Lactococcus lactis subsp. Cremori, B.longum, B. adolescentis and B. lactis (P<0.05 or P<0.01). The oxalate-degrading rate of B. lactis was the highest (29.03%), and that of Enterococcaceae faecium was the lowest (0.23%). The correlation analysis revealed that there was no significant correlation between times of proliferation and oxalate-degrading rates (r=0.435 7, P=0.208 2). Conclusion All of the 10 strains of lactic acid bacteria for yoghourt fermentation have the ability of oxalate degrading, and there is no correlation between lactic acid bacteria proliferation and oxalate degradation.
