CAJ | 학술논문

已分离鉴定的无色杆菌属(Achromobacter sp.)菌株MGT3对孔雀绿染料具有强脱色作用,对其产酶脱色能力进行初步研究表明,该菌株不需要孔雀绿作为底物即可产脱色酶,即脱色酶的产生不需要底物的诱导;其胞内和胞外都存在脱色能力强的脱色酶.产胞外脱色酶的条件优化结果显示培养基的组成对该菌株产胞外酶的能力有很大影响.采用单因素结合正交试验优化产酶培养基结果表明,培养基的最佳组成成分为0.5%蔗糖、2.5%尿素、0.0025%KH_2PO_4;该菌株在培养基初始pH为9、温度为35 ℃时产脱色酶效率高,对孔雀绿脱色率高.
이분리감정적무색간균속(Achromobacter sp.)균주MGT3대공작록염료구유강탈색작용,대기산매탈색능력진행초보연구표명,해균주불수요공작록작위저물즉가산탈색매,즉탈색매적산생불수요저물적유도;기포내화포외도존재탈색능력강적탈색매.산포외탈색매적조건우화결과현시배양기적조성대해균주산포외매적능력유흔대영향.채용단인소결합정교시험우화산매배양기결과표명,배양기적최가조성성분위0.5%자당、2.5%뇨소、0.0025%KH_2PO_4;해균주재배양기초시pH위9、온도위35 ℃시산탈색매효솔고,대공작록탈색솔고.
The Achromobacter sp. MGT3 can highly decolorize malachite green. The results on its enzyme producing capability showed that the production of decoloring-enzymes did not require the induction of substrate, and the decoloring-enzymes not only existed inside the cells, but also outside the cells. The results on the exoenzyme producing conditions showed that the compositions of medium had great influence on enzyme producing capability of MGT3. Single-factor and orthogonal experiment optimizing the exoenzyme producing medium showed that the optimized compositions of medium were 0.5% sucrose, 2.5% urea and 0.0025% KH_2PO_4. The optimum pH for decolorizing malachite green was 9, and the temperature was 35 ℃.