CAJ | 학술논문

目的揭示东北梅花鹿卵母细胞及其细胞器的发育规律.方法根据梅花鹿卵泡直径大小、透明带的形成、卵泡腔的出现时间把非发情期和发情期卵泡系统分为:原始卵泡、生长卵泡和成熟卵泡3个时期,并利用电镜、目镜测微尺和显微照相技术对各期卵母细胞发育过程中细胞器进行观察(每类卵母细胞观察6～8个).结果 1.原始卵泡和生长卵泡前期:线粒体、高尔基体、滑面内质网(SER)、皮质颗粒等细胞器数量不断增加,并逐渐移向皮质区.2.生长卵泡后期和成熟卵泡期:高尔基体及粗面内质网(RER)消失,皮质颗粒开始在质膜下呈线性排列,线粒体向胞质中央区扩散且少嵴圆形线粒体一律转变为带帽线粒体,核仁发生致密化.3.短而粗的微绒毛从初级卵泡卵母细胞开始出现,到次级卵泡卵母细胞时变得密集而细长,三级卵泡期则开始缩短变粗,部分退出透明带.结论在卵母细胞发育过程中,线粒体的形态、数量及分布的变化与细胞的代谢水平、增殖及分化密切相关;皮质颗粒与高尔基体基本无关联,但却与滑面内质网关系密切;核仁既是RNA的合成场所又是其贮存场所,同时RNA致密化也成为减数分裂恢复的前提.
목적 게시동북매화록란모세포급기세포기적발육규률.방법 근거매화록란포직경대소、투명대적형성、란포강적출현시간파비발정기화발정기란포계통분위:원시란포、생장란포화성숙란포3개시기,병이용전경、목경측미척화현미조상기술대각기란모세포발육과정중세포기진행관찰(매류란모세포관찰6～8개).결과 1.원시란포화생장란포전기:선립체、고이기체、활면내질망(SER)、피질과립등세포기수량불단증가,병축점이향피질구.2.생장란포후기화성숙란포기:고이기체급조면내질망(RER)소실,피질과립개시재질막하정선성배렬,선립체향포질중앙구확산차소척원형선립체일률전변위대모선립체,핵인발생치밀화.3.단이조적미융모종초급란포란모세포개시출현,도차급란포란모세포시변득밀집이세장,삼급란포기칙개시축단변조,부분퇴출투명대.결론 재란모세포발육과정중,선립체적형태、수량급분포적변화여세포적대사수평、증식급분화밀절상관;피질과립여고이기체기본무관련,단각여활면내질망관계밀절;핵인기시RNA적합성장소우시기저존장소,동시RNA치밀화야성위감수분렬회복적전제.
Objective Revealing the developmental regulation of Cervus nippon's oocyte and organelles. Methods In the experiment,follicle systems during both estrum and non- estrum were divided into the primordial follicle,growing follicle and mature follicle according to the Cervus nippon's follicle diameter size,formation of zonapellucida,appearance time of follicular cavity.At the same time,observations on cytoplasmic organelles in development of oocytes were conducted with electron microscopic,eyepiece micrometer and photomicrographic technique(The number of every oocyte observed is 6-8). Results In the primordial follicle and early growing follicle phase,the quantity of mitochondria,golgi apparatus,smooth endoplasmic reticulum and cortical granules increased gradually and all organelles moved to the cortical area.However,in the late growing follicle and mature follicle phase,Golgi apparatus and rough endoplasmic reticulum disappeared,cortical granules began to arrange themselves in line beneath the plasma membrane of the oocyte,mitochondrias dispersed toward the central region of cytoplasm,and almost all the round mitochondria with rare cristae turned into hooded ones, and nucleus compaction occurred.In addition,the short and thick microvilli began to appear from the primary follicle ovocyte,become intensive and slender when secondary follicle's ovocyte;It's until tertiary follicle's ovocyte,microvilli started to shorten and become coarse,and even parts of them contract from the zona pellucida gradually.Conclusion In the development of oocytes, the changes of type,quantity and distribution on mitochondria has a close relation with cells at proliferation,differentiation and metabolism level.Cortical granule has no association with golgi apparatus basically,but smooth endoplasmic reticulum(SER).The nuclei are the sites of RNA synthesis and warehouses,and its densification is the premise of meiosis recovery.