中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
15期
2692-2695
,共4页
朱伟民%陆伟%韩云%周可%欧阳侃%彭亮权%冯文哲%李皓%何春耒%王大平%江捍平
硃偉民%陸偉%韓雲%週可%歐暘侃%彭亮權%馮文哲%李皓%何春耒%王大平%江捍平
주위민%륙위%한운%주가%구양간%팽량권%풍문철%리호%하춘뢰%왕대평%강한평
骨关节炎%基因表达%cDNA微阵列%抗体%单克隆%软骨组织工程
骨關節炎%基因錶達%cDNA微陣列%抗體%單剋隆%軟骨組織工程
골관절염%기인표체%cDNA미진렬%항체%단극륭%연골조직공정
背景:原发性骨关节炎被确认为是一种多基因疾病.采用cDNA微阵列技术,从基因表达水平在同一载体上同时进行多基因检测,从而考察骨关节炎的滑膜与软骨基因表达类型,有助于进一步认识骨关节炎相关发病机制及为基因治疗提供依据.目的:通过比较骨性关节炎和正常关节的软骨与滑膜的基因谱变化,筛选出骨性关节相关的差异表达基因,并探讨其在骨性关节炎发病机制中的意义及骨性关节炎的基因多态性.方法:Wistar大鼠24只,随机分为模型组和对照组,每组12只.提取模型组和对照组的膝关节软骨与滑膜细胞,提取总RNA.用含588个基因的cDNA芯片进行微阵列基因检测,数据进行基因差异表达和聚类分析.结果与结论:骨性关节炎大鼠及正常大鼠软骨及滑膜的基因差异表达分析结果显示,骨关节炎大鼠软骨与滑膜中的差异表达基因共有82个,其中上调的基因有27个,下调的基因有55个.基因芯片技术能有效地筛选出骨关节炎差异表达的基因并可发现新的相关基因.骨关节炎的发病涉及多种基因的表达异常,筛选到的差异表达基因将为进一步研究骨关节炎发病机制和致病相关基因功能提供理论依据.
揹景:原髮性骨關節炎被確認為是一種多基因疾病.採用cDNA微陣列技術,從基因錶達水平在同一載體上同時進行多基因檢測,從而攷察骨關節炎的滑膜與軟骨基因錶達類型,有助于進一步認識骨關節炎相關髮病機製及為基因治療提供依據.目的:通過比較骨性關節炎和正常關節的軟骨與滑膜的基因譜變化,篩選齣骨性關節相關的差異錶達基因,併探討其在骨性關節炎髮病機製中的意義及骨性關節炎的基因多態性.方法:Wistar大鼠24隻,隨機分為模型組和對照組,每組12隻.提取模型組和對照組的膝關節軟骨與滑膜細胞,提取總RNA.用含588箇基因的cDNA芯片進行微陣列基因檢測,數據進行基因差異錶達和聚類分析.結果與結論:骨性關節炎大鼠及正常大鼠軟骨及滑膜的基因差異錶達分析結果顯示,骨關節炎大鼠軟骨與滑膜中的差異錶達基因共有82箇,其中上調的基因有27箇,下調的基因有55箇.基因芯片技術能有效地篩選齣骨關節炎差異錶達的基因併可髮現新的相關基因.骨關節炎的髮病涉及多種基因的錶達異常,篩選到的差異錶達基因將為進一步研究骨關節炎髮病機製和緻病相關基因功能提供理論依據.
배경:원발성골관절염피학인위시일충다기인질병.채용cDNA미진렬기술,종기인표체수평재동일재체상동시진행다기인검측,종이고찰골관절염적활막여연골기인표체류형,유조우진일보인식골관절염상관발병궤제급위기인치료제공의거.목적:통과비교골성관절염화정상관절적연골여활막적기인보변화,사선출골성관절상관적차이표체기인,병탐토기재골성관절염발병궤제중적의의급골성관절염적기인다태성.방법:Wistar대서24지,수궤분위모형조화대조조,매조12지.제취모형조화대조조적슬관절연골여활막세포,제취총RNA.용함588개기인적cDNA심편진행미진렬기인검측,수거진행기인차이표체화취류분석.결과여결론:골성관절염대서급정상대서연골급활막적기인차이표체분석결과현시,골관절염대서연골여활막중적차이표체기인공유82개,기중상조적기인유27개,하조적기인유55개.기인심편기술능유효지사선출골관절염차이표체적기인병가발현신적상관기인.골관절염적발병섭급다충기인적표체이상,사선도적차이표체기인장위진일보연구골관절염발병궤제화치병상관기인공능제공이론의거.
BACKGROUND:Primary osteoarthritis (OA) is a multigenic disease.Using cDNA microarray,multigenic detection is performed on one carrier to explore gene expression type of cartilage and synovium,which provides evidence for related mechanism and gene therapy.OBJECTIVE:To compare synovium and cartilage gene expression profile of OA rats and to evaluate the potential OA related discriminating genes and their roles in pathogenesis of OA.METHODS:A total of 24 male Wistar rats were randomly divided into model and control groups with 12 animals in each group.Synovial and cartilage tissue were obtained from two groups.Total RNA was extracted from the tissues.cDNA microarray chips were used to identify gene expression profile.Differential expression and clustering analysis were performed.RESULTS AND CONCLUSION:Total gene difierential expression in synovial tissue and cartilage of OA showed a high relativity.Among the target and cartilage genes,82 differentially expressed genes.were identified in OA rats,including 27 up-regulated genes and 55 down-regulated genes.Gene microarray technique is effective for screening associated genes,which helps to understand the pathogenesis of OA,and find the new genes associated with inflammation.OA is an immunogenic disorder with many abnormal expression genes.Genes screened from the target can provide important information for further study and genetic treatment of OA.
