中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2010年
4期
393-397
,共5页
余勤%白月双%林洁%盛丽先%董勤%郭莹%宣晓波
餘勤%白月雙%林潔%盛麗先%董勤%郭瑩%宣曉波
여근%백월쌍%림길%성려선%동근%곽형%선효파
黄芪注射液%间质干细胞%脊髓损伤%移植
黃芪註射液%間質榦細胞%脊髓損傷%移植
황기주사액%간질간세포%척수손상%이식
Astragalus Injection%mesenchymal stem cells%spinal cord injury%transplantation
目的 研究黄芪注射液与间质干细胞联合应用对大鼠脊髓损伤修复的影响.方法 Wistar大鼠120只随机分成假手术组、模型组、PBS液对照组、黄芪注射液对照组、大鼠骨髓间质干细胞(rMSCs)移植治疗组和rMSCs移植加黄芪注射液治疗组.造模后3 d治疗组经脊髓局部注射rMSCs和脊髓局部注射rMSCs同时加用黄芪注射液腹腔注射,对照组则分别注射PBS液和黄芪注射液.移植后7、14、21、28天分别行(Basso Beattie Bresnahan,BBB)评分法检测大鼠神经功能恢复情况,进一步取脊髓组织作病理学检查,并应用双标免疫组化检测5-溴-2脱氧尿嘧啶核苷(BrdU)标记rMSCs的胶质纤维酸性蛋白(GFAP)和神经元中丝蛋白(NF-M)表达情况.结果 治疗组较对照组损伤脊髓修复明显,神经功能评分显著提高(P＜0.05).rMSCs移植加黄芪注射液治疗组在不同时间点的神经功能评分都高于rMSCs移植治疗组.脊髓组织病理切片显示rMSCs移植加黄芪注射液治疗组较rMSCs移植治疗组组织水肿和炎性细胞浸润减轻更明显,胶质细胞增生更活跃.双标免疫组化显示移植的rMSCs在宿主脊髓中存活,从第7 d开始即有GFAP和NF-M表达并向损伤部位迁移.rMSCs加黄芪注射液治疗组中GFAP、NF-M阳性细胞数目均较rMSCs移植治疗组增多(P＜0.05).结论 rMSCs移植是治疗脊髓损伤的有效方法;黄芪注射液在体内有诱导rMSCs向神经细胞分化的能力,并有协同rMSCs促进大鼠脊髓损伤修复的作用.
目的 研究黃芪註射液與間質榦細胞聯閤應用對大鼠脊髓損傷脩複的影響.方法 Wistar大鼠120隻隨機分成假手術組、模型組、PBS液對照組、黃芪註射液對照組、大鼠骨髓間質榦細胞(rMSCs)移植治療組和rMSCs移植加黃芪註射液治療組.造模後3 d治療組經脊髓跼部註射rMSCs和脊髓跼部註射rMSCs同時加用黃芪註射液腹腔註射,對照組則分彆註射PBS液和黃芪註射液.移植後7、14、21、28天分彆行(Basso Beattie Bresnahan,BBB)評分法檢測大鼠神經功能恢複情況,進一步取脊髓組織作病理學檢查,併應用雙標免疫組化檢測5-溴-2脫氧尿嘧啶覈苷(BrdU)標記rMSCs的膠質纖維痠性蛋白(GFAP)和神經元中絲蛋白(NF-M)錶達情況.結果 治療組較對照組損傷脊髓脩複明顯,神經功能評分顯著提高(P＜0.05).rMSCs移植加黃芪註射液治療組在不同時間點的神經功能評分都高于rMSCs移植治療組.脊髓組織病理切片顯示rMSCs移植加黃芪註射液治療組較rMSCs移植治療組組織水腫和炎性細胞浸潤減輕更明顯,膠質細胞增生更活躍.雙標免疫組化顯示移植的rMSCs在宿主脊髓中存活,從第7 d開始即有GFAP和NF-M錶達併嚮損傷部位遷移.rMSCs加黃芪註射液治療組中GFAP、NF-M暘性細胞數目均較rMSCs移植治療組增多(P＜0.05).結論 rMSCs移植是治療脊髓損傷的有效方法;黃芪註射液在體內有誘導rMSCs嚮神經細胞分化的能力,併有協同rMSCs促進大鼠脊髓損傷脩複的作用.
목적 연구황기주사액여간질간세포연합응용대대서척수손상수복적영향.방법 Wistar대서120지수궤분성가수술조、모형조、PBS액대조조、황기주사액대조조、대서골수간질간세포(rMSCs)이식치료조화rMSCs이식가황기주사액치료조.조모후3 d치료조경척수국부주사rMSCs화척수국부주사rMSCs동시가용황기주사액복강주사,대조조칙분별주사PBS액화황기주사액.이식후7、14、21、28천분별행(Basso Beattie Bresnahan,BBB)평분법검측대서신경공능회복정황,진일보취척수조직작병이학검사,병응용쌍표면역조화검측5-추-2탈양뇨밀정핵감(BrdU)표기rMSCs적효질섬유산성단백(GFAP)화신경원중사단백(NF-M)표체정황.결과 치료조교대조조손상척수수복명현,신경공능평분현저제고(P＜0.05).rMSCs이식가황기주사액치료조재불동시간점적신경공능평분도고우rMSCs이식치료조.척수조직병리절편현시rMSCs이식가황기주사액치료조교rMSCs이식치료조조직수종화염성세포침윤감경경명현,효질세포증생경활약.쌍표면역조화현시이식적rMSCs재숙주척수중존활,종제7 d개시즉유GFAP화NF-M표체병향손상부위천이.rMSCs가황기주사액치료조중GFAP、NF-M양성세포수목균교rMSCs이식치료조증다(P＜0.05).결론 rMSCs이식시치료척수손상적유효방법;황기주사액재체내유유도rMSCs향신경세포분화적능력,병유협동rMSCs촉진대서척수손상수복적작용.
Objective To observe the effect of Astragalus Injection(ASI)combined with rat's mesenchymal stem cells transplantation(rMSCs)for repairing spinal cord injury in rats.Methods One hundred and twenty Wistar rats were randomly divided into 6 groups,named respectively by letters from A-F.they were treated respectively with none,PBS solution,ASI,rMSCs,and ASI + rMSCs,with the ASI administered via intraperitoneal injection and the rMSCs given by local injection to the spinal cord,on the 3rd day of operation.The condition of nerve function recovery was assessed on the 7th,14th,21st and 28th day after treatment by scoring according to the Basso-Beattie-Bresnahan(BBB)Iocomotor rating scale.Besides,the pathological change of the injured spinal cord was observed and expressions of glial fibriallary acidic protein(GFAP)and neurofilament-M(NF-M)in the BrdU-Iabeled rMSCs in the spinal cord tissue were examined by immune histochemistry.Results The recovery of spinal cord in Group D,E and F was better than that in Group B and C,showing higher BBB scores.As compared with Group E,Group F showed a higher score of nerve function and a milder inflammatory cell infiltration with lessened tissue edema in the spinal cord and more active proliferation of gliacyte.Double-labelled immunohistochemical examination showed that the transplanted rMSCs were alive in the host's spinal cord,revealing the expressions of GFAP and NF-M from the 7th day after transplantation,which were migrating to the injured site.The amount of GFAP and NF-M positive cells in Group F was much more than that in Group E(P＜0.05)Conclusion Transplantation of rMSCs is an effective method in treatment of the spinal cord injury; ASI has the capacity for inducing rMSCs differentiated into neurons,and could synergize with rMSCs to promote the repairing of the spinal cord from injury.
