国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2010年
23期
1409-1412
,共4页
哮喘%IL-12%基因多态性%蛋白表达%IgE
哮喘%IL-12%基因多態性%蛋白錶達%IgE
효천%IL-12%기인다태성%단백표체%IgE
Asthma%Interleukin-12%Polymorphism%Protein expression%IgE
目的 探讨白介素12(IL-12)-1188(A＞C)的基因多态性与中国北方成人支气管哮喘(简称哮喘)的相关性,并探讨其对血清总IgE水平的关系及对蛋白表达的影响.方法 采用特异序列引物聚合酶链反应方法检测65例哮喘患者和52名健康成人IL-12-1188位点基因型,比较两组IL-12-1188位点的基因型和等位基因分布频率.同时采用酶联免疫吸附试验检测不同基因型的研究对象的血清总IgE水平及蛋白表达的水平.结果 哮喘组和健康组3种基因型AA,AC,CC的基因分布频率分别为:29.2%,58.5%,12.3%; 53.9%,46.1%,0%.两组基因型分布频率的差异有统计学意义(P＜0.01),哮喘组和健康组A和C的等位基因分布频率分别是58.45%,41.55%;76.95%23.05%.两组等位基因的分布频率的差异有统计学意义(P＜0.05).另外,血清总IgE水平哮喘组(472.15±23.11)μg/L高于正常对照组(65.66±12.42) μg/L,差异有统计学意义(P＜0.01);而蛋白表达的水平哮喘组(2.15±0.89)μg/L低于正常对照组(3.77±1.01)μg/L,差异有统计学意义(P＜0.05).结论 研究表明IL-12-1188(A/C)的基因多态性与中国北方成人哮喘的发生有关,并且该位点的基因多态性可引起血清总IgE水平及蛋白表达水平的变化.
目的 探討白介素12(IL-12)-1188(A＞C)的基因多態性與中國北方成人支氣管哮喘(簡稱哮喘)的相關性,併探討其對血清總IgE水平的關繫及對蛋白錶達的影響.方法 採用特異序列引物聚閤酶鏈反應方法檢測65例哮喘患者和52名健康成人IL-12-1188位點基因型,比較兩組IL-12-1188位點的基因型和等位基因分佈頻率.同時採用酶聯免疫吸附試驗檢測不同基因型的研究對象的血清總IgE水平及蛋白錶達的水平.結果 哮喘組和健康組3種基因型AA,AC,CC的基因分佈頻率分彆為:29.2%,58.5%,12.3%; 53.9%,46.1%,0%.兩組基因型分佈頻率的差異有統計學意義(P＜0.01),哮喘組和健康組A和C的等位基因分佈頻率分彆是58.45%,41.55%;76.95%23.05%.兩組等位基因的分佈頻率的差異有統計學意義(P＜0.05).另外,血清總IgE水平哮喘組(472.15±23.11)μg/L高于正常對照組(65.66±12.42) μg/L,差異有統計學意義(P＜0.01);而蛋白錶達的水平哮喘組(2.15±0.89)μg/L低于正常對照組(3.77±1.01)μg/L,差異有統計學意義(P＜0.05).結論 研究錶明IL-12-1188(A/C)的基因多態性與中國北方成人哮喘的髮生有關,併且該位點的基因多態性可引起血清總IgE水平及蛋白錶達水平的變化.
목적 탐토백개소12(IL-12)-1188(A＞C)적기인다태성여중국북방성인지기관효천(간칭효천)적상관성,병탐토기대혈청총IgE수평적관계급대단백표체적영향.방법 채용특이서렬인물취합매련반응방법검측65례효천환자화52명건강성인IL-12-1188위점기인형,비교량조IL-12-1188위점적기인형화등위기인분포빈솔.동시채용매련면역흡부시험검측불동기인형적연구대상적혈청총IgE수평급단백표체적수평.결과 효천조화건강조3충기인형AA,AC,CC적기인분포빈솔분별위:29.2%,58.5%,12.3%; 53.9%,46.1%,0%.량조기인형분포빈솔적차이유통계학의의(P＜0.01),효천조화건강조A화C적등위기인분포빈솔분별시58.45%,41.55%;76.95%23.05%.량조등위기인적분포빈솔적차이유통계학의의(P＜0.05).령외,혈청총IgE수평효천조(472.15±23.11)μg/L고우정상대조조(65.66±12.42) μg/L,차이유통계학의의(P＜0.01);이단백표체적수평효천조(2.15±0.89)μg/L저우정상대조조(3.77±1.01)μg/L,차이유통계학의의(P＜0.05).결론 연구표명IL-12-1188(A/C)적기인다태성여중국북방성인효천적발생유관,병차해위점적기인다태성가인기혈청총IgE수평급단백표체수평적변화.
Objective To evaluate the possible association between the IL-12-1188 polymorphism and asthmatic adult in the north of China,and to investigate the relationship between polymorphism and serum IgE level in them,and an effect on its protein expression. Methods The study was conducted in two different groups:asthmatic patients ( n =65)and healthy adults( n = 52). The IL-12-1188 polymorphism was genotyped by polymerase chain reaction-restriction amplification with sequence-specific primers. And Serum total IgE level and protein of subjects with different genotypes was determined by enzyme linked immunosorbent assay. Results Genotypes ftequencies of AA, AC, and CC were 29.2 %, 58.5 %, 12.3 % and allels frequencies of A and C were 58.46%, 41.54% in the asthmatic group. However, genotypes frequencies of AA, AC, and CC were 53.8% ,46.2%, 0% and allels frequencies of A,C were 76.92%,23.08% in the healthy group. There are significant difference in the distribution of genotypes and alleles frequencies between the two groups. And serum total IgE level in asthmatic group(472. 15 ± 23.11 ) μg/L was higher than control group(65.66± 12.42) μg/L, ( P ＜0.01 ＝. But the different genotypes of IL-12 1188 (A/ C) had an effecton on its protein expression level. The protein level from the patients with (2. 15±0.89) μg/L,was lower than patients with (3.77± 1.01 ) μg/L ( P ＜0. 05). Conclusions There is polymorphism of IL-12-1188 sites, which may be correlated to the etiology of adult asthma in north of China, but may related to the increase of total serum IgE level and the decreass of protein expression level.
