中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
8期
574-576
,共3页
孙青苗%赵琰%蔡林%张建中
孫青苗%趙琰%蔡林%張建中
손청묘%조염%채림%장건중
结核,皮肤%Toll样受体2%Toll样受体4%Toll样受体9
結覈,皮膚%Toll樣受體2%Toll樣受體4%Toll樣受體9
결핵,피부%Toll양수체2%Toll양수체4%Toll양수체9
Tuberculosis,cutaneous%Toll-like receptor 2%Toll-like receptor 4%Toll-like receptor 9
目的 研究Toll样受体2、4和9(TLR-2、TLR-4、TLR-9)在寻常狼疮和疣状皮肤结核皮损中的表达,探讨其在皮肤结核发病中的作用.方法 经临床与病理确诊的皮肤结核(包括寻常狼疮和疣状皮肤结核)患者皮损标本18份,斑块状银屑病皮损15份为阳性对照,色素痣边缘正常皮肤10份为阴性对照.用免疫组化法观察TLR-2、TLR-4、TLR-9的表达情况.采用SPSS 13.0统计软件进行统计学分析,组间比较行t检验.结果 TLR-2在正常皮肤表达于除基底层外的表皮全层,而在皮肤结核和银屑病皮损表达于表皮的中上层,3组的表达强度均为高表达(A值分别为0.28±0.03、0.25±0.04和0.25±0.05),3组差异无统计学意义(P＞0.05).TLR-4在正常皮肤和银屑病呈低或无表达(A值分别为0.07±0.09和0.02±0.05),而皮肤结核(0.16±0.07)呈低表达,其表达强度高于正常皮肤(t=2.58,P＜0.05)和银屑病皮损(t=6.24,P＜ 0.01).TLR-9在正常皮肤和皮肤结核表达于表皮全层、附属器及血管壁,而皮肤结核的表达强度(A值为0.25±0.05)高于正常皮肤(0.19±0.05),两组比较,t=2.88,P＜ 0.05.结论 TLR-2、TLR-4和TLR-9在皮肤结核皮损中均存在表达,其中TLR-4和TLR-9的表达高于正常皮肤,提示其在皮肤结核的免疫应答中可能起一定的作用.
目的 研究Toll樣受體2、4和9(TLR-2、TLR-4、TLR-9)在尋常狼瘡和疣狀皮膚結覈皮損中的錶達,探討其在皮膚結覈髮病中的作用.方法 經臨床與病理確診的皮膚結覈(包括尋常狼瘡和疣狀皮膚結覈)患者皮損標本18份,斑塊狀銀屑病皮損15份為暘性對照,色素痣邊緣正常皮膚10份為陰性對照.用免疫組化法觀察TLR-2、TLR-4、TLR-9的錶達情況.採用SPSS 13.0統計軟件進行統計學分析,組間比較行t檢驗.結果 TLR-2在正常皮膚錶達于除基底層外的錶皮全層,而在皮膚結覈和銀屑病皮損錶達于錶皮的中上層,3組的錶達彊度均為高錶達(A值分彆為0.28±0.03、0.25±0.04和0.25±0.05),3組差異無統計學意義(P＞0.05).TLR-4在正常皮膚和銀屑病呈低或無錶達(A值分彆為0.07±0.09和0.02±0.05),而皮膚結覈(0.16±0.07)呈低錶達,其錶達彊度高于正常皮膚(t=2.58,P＜0.05)和銀屑病皮損(t=6.24,P＜ 0.01).TLR-9在正常皮膚和皮膚結覈錶達于錶皮全層、附屬器及血管壁,而皮膚結覈的錶達彊度(A值為0.25±0.05)高于正常皮膚(0.19±0.05),兩組比較,t=2.88,P＜ 0.05.結論 TLR-2、TLR-4和TLR-9在皮膚結覈皮損中均存在錶達,其中TLR-4和TLR-9的錶達高于正常皮膚,提示其在皮膚結覈的免疫應答中可能起一定的作用.
목적 연구Toll양수체2、4화9(TLR-2、TLR-4、TLR-9)재심상랑창화우상피부결핵피손중적표체,탐토기재피부결핵발병중적작용.방법 경림상여병리학진적피부결핵(포괄심상랑창화우상피부결핵)환자피손표본18빈,반괴상은설병피손15빈위양성대조,색소지변연정상피부10빈위음성대조.용면역조화법관찰TLR-2、TLR-4、TLR-9적표체정황.채용SPSS 13.0통계연건진행통계학분석,조간비교행t검험.결과 TLR-2재정상피부표체우제기저층외적표피전층,이재피부결핵화은설병피손표체우표피적중상층,3조적표체강도균위고표체(A치분별위0.28±0.03、0.25±0.04화0.25±0.05),3조차이무통계학의의(P＞0.05).TLR-4재정상피부화은설병정저혹무표체(A치분별위0.07±0.09화0.02±0.05),이피부결핵(0.16±0.07)정저표체,기표체강도고우정상피부(t=2.58,P＜0.05)화은설병피손(t=6.24,P＜ 0.01).TLR-9재정상피부화피부결핵표체우표피전층、부속기급혈관벽,이피부결핵적표체강도(A치위0.25±0.05)고우정상피부(0.19±0.05),량조비교,t=2.88,P＜ 0.05.결론 TLR-2、TLR-4화TLR-9재피부결핵피손중균존재표체,기중TLR-4화TLR-9적표체고우정상피부,제시기재피부결핵적면역응답중가능기일정적작용.
Objective To observe the expression of Toll-like receptors (TLRs) 2,4 and 9 in lesions of lupus vulgaris and tuberculosis verrucosa cutis,and to evaluate the possible roles of these receptors in the pathogenesis of cutaneous tuberculosis.Methods Skin specimens were obtained from the lesions of 18 patients with clinically and pathologically diagnosed lupus vulgaris or tuberculosis verrucosa cutis,15 patients with plaque-type psoriasis (positive control),and from perilesional normal skin of 10 patients with pigmented nevi (negative control).Immunohistochemistry was used to detect the expression of TLRs 2,4 and 9 in these specimens,and the expression intensity was expressed as absorbence values.Data were analysed by using the SPSS 13.0 software,and t test was conducted to assess the differences between the three groups of specimens.Results TLR-2 was observed mainly in the middle and upper layer of epidermis in lesions of cutaneous tuberculosis and psoriasis,and in the whole epidermis except the basal layer in normal skin,with no significant difference in the expression intensity between the three groups of specimens (0.25 ± 0.04 vs.0.25 ± 0.05 vs.0.28 ± 0.03,P ＞ 0.05).TLR-4 was weakly expressed in cutaneous tuberculosis lesions,absent or slightly expressed in normal skin and psoriatic lesions,and the expression level of TLR-4 was significantly higher in cutaneous tuberculosis lesions than in normal skin and psoriatic lesions (0.16 ± 0.07 vs.0.07 ± 0.09 and 0.02± 0.05,t =2.58,6.24,P ＜ 0.01 and 0.05).TLR-9 was expressed in the whole epidermal layer,including appendages and vascular walls,of cutaneous tuberculosis and normal skin,with a significant increase in cutaneous tuberculosis than in the normal skin (0.25 ± 0.05 vs.0.19 ± 0.05,t =2.88,P＜ 0.05).Conclusions TLRs 2,4 and 9 are all expressed in cutaneous tuberculosis losious,and the expression intensity of TLR-4 and TLR-9 is higher in cutaneous tuberculosis lesions than in normal skin,hinting that TLR-4 and TLR-9 play a certain role in the immune response of cutaneous tuberculosis.
