CAJ | 학술논문

目的探讨VEGFR-3信号途径在甲状腺癌淋巴道转移中的作用.方法 RT-PCR检测甲状腺癌组织及正常甲状腺组织VEGFR-3的表达水平;免疫组织化学染色法检测甲状腺癌组织及正常甲状腺组织VEGFR-3的表达,测定淋巴管密度.结果甲状腺癌组织中VEGFR-3的表达率为100%,高于正常组织组(P＜0.05);LVD在癌组织中显著高于正常组织对照组,且淋巴结有癌转移组(N+)的LVD显著高于淋巴结无转移组(NO)(P＜0.05).结论甲状腺癌组织中高表达VEGFR-3,表达强度随淋巴结转移的严重度增加而增强,VEGFR-3受体介导的信号传导途径可能是导致甲状腺癌出现淋巴结转移的重要因素.
목적 탐토VEGFR-3신호도경재갑상선암림파도전이중적작용.방법 RT-PCR검측갑상선암조직급정상갑상선조직VEGFR-3적표체수평;면역조직화학염색법검측갑상선암조직급정상갑상선조직VEGFR-3적표체,측정림파관밀도.결과 갑상선암조직중VEGFR-3적표체솔위100%,고우정상조직조(P＜0.05);LVD재암조직중현저고우정상조직대조조,차림파결유암전이조(N+)적LVD현저고우림파결무전이조(NO)(P＜0.05).결론 갑상선암조직중고표체VEGFR-3,표체강도수림파결전이적엄중도증가이증강,VEGFR-3수체개도적신호전도도경가능시도치갑상선암출현림파결전이적중요인소.
Objective To investigate the involvement of VEGFR-3 signaling pathway in lymphatic metastasis of thyroid cancer. Methods The expression of VEGFR-3 mRNA in thyroid carcinoma and normal thyroid tissue was detected by RT-PCR. The expression of VEGFR-3 mRNA and the lymphatic vessel density (LVD) in thyroid carcinoma and normal thyroid tissue was measured by immunohistochemical staining. Results All thyroid cancer samples expressed VEGFR-3 and the expression rate of VEGFR-3 in thyroid carcinoma was significantly higher than that in the normal thyroid group ( P ＜ 0. 05 ). LVD in thyroid cancer was significantly higher than that in normal control. LVD with lymph nodes metastasis ( N + ) was significantly higher than that without lymph node metastasis (NO) (P ＜ 0. 05). Conclusions There is upregulatin of VEGFR-3 expression in thyroid carcinoma. The higher the expression of VEGFR-3, the severer of lymph node metastasis. VEGFR-3 receptor-mediated signal transduction pathway might be an important factor of thyroid cancer lymph node metastasis.