中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2011年
2期
95-98
,共4页
庞庆丰%徐文莉%何俊%陈华林
龐慶豐%徐文莉%何俊%陳華林
방경봉%서문리%하준%진화림
血红素加氧酶-1%内毒素血症%谷氨酰胺%肠道%炎症反应
血紅素加氧酶-1%內毒素血癥%穀氨酰胺%腸道%炎癥反應
혈홍소가양매-1%내독소혈증%곡안선알%장도%염증반응
Heme oxygenase-1%Endotoxemia%Glutamine%Intestinal tract%Inflammation
目的 探讨谷氨酰胺(Glu)对内毒素血症大鼠肠道损伤的保护作用以及对血红素加氧酶-1(HO-1)表达的影响.方法 按随机数字表法将32只雄性SD 大鼠分为正常对照组、模型组、Glu组和Glu+锌原卟啉(ZnPP)组,每组8只.腹腔注射内毒素脂多糖(LPS)10 mg/kg制备内毒素血症动物模型.Glu组注射LPS前12 h灌胃Glu 1 g/kg;Glu+ZnPP组注射LPS前12 h灌胃Glu 1 g/kg,注射LPS前1 h静脉注射ZnPP 10 mmol/kg.术后12 h取回肠组织,测定髓过氧化物酶(MPO)活性及肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)含量,并进行肠组织学评分;用免疫组化法检测回肠组织HO-1表达.结果 与正常对照组比较,模型组回肠组织学评分、MPO活性及TNF-α、IL-10含量显著升高[组织学评分(分):3.3±0.4比1.1±0.6,MPO活性(U/g):0.40±0.08比0.26±0.07,TNF-α含量(ng/g):25.2±6.9比6.5±2.8,IL-10含量(ng/g):27.6±10.2比5.7±2.9,均P<0.01];HO-1表达较低.与模型组比较,Glu组组织学评分、MPO活性和TNF-α含量明显减低[组织学评分(分):1.6±0.5比3.3±0.4,MPO活性(U/g):0.25±0.05比0.40±0.08,TNF-α含量(ng/g):13.4±3.2比25.2±6.9,均P<0.01],IL-10含量(ng/g)显著升高(47.3±5.5比27.6±10.2,P<0.01),HO-1表达明显增加.Glu+ZnPP组与模型组各指标比较差异无统计学意义.结论 Glu能明显增强内毒素血症大鼠肠组织HO-1表达,明显减轻肠道炎症反应,从而保护肠黏膜.
目的 探討穀氨酰胺(Glu)對內毒素血癥大鼠腸道損傷的保護作用以及對血紅素加氧酶-1(HO-1)錶達的影響.方法 按隨機數字錶法將32隻雄性SD 大鼠分為正常對照組、模型組、Glu組和Glu+鋅原卟啉(ZnPP)組,每組8隻.腹腔註射內毒素脂多糖(LPS)10 mg/kg製備內毒素血癥動物模型.Glu組註射LPS前12 h灌胃Glu 1 g/kg;Glu+ZnPP組註射LPS前12 h灌胃Glu 1 g/kg,註射LPS前1 h靜脈註射ZnPP 10 mmol/kg.術後12 h取迴腸組織,測定髓過氧化物酶(MPO)活性及腫瘤壞死因子-α(TNF-α)、白細胞介素-10(IL-10)含量,併進行腸組織學評分;用免疫組化法檢測迴腸組織HO-1錶達.結果 與正常對照組比較,模型組迴腸組織學評分、MPO活性及TNF-α、IL-10含量顯著升高[組織學評分(分):3.3±0.4比1.1±0.6,MPO活性(U/g):0.40±0.08比0.26±0.07,TNF-α含量(ng/g):25.2±6.9比6.5±2.8,IL-10含量(ng/g):27.6±10.2比5.7±2.9,均P<0.01];HO-1錶達較低.與模型組比較,Glu組組織學評分、MPO活性和TNF-α含量明顯減低[組織學評分(分):1.6±0.5比3.3±0.4,MPO活性(U/g):0.25±0.05比0.40±0.08,TNF-α含量(ng/g):13.4±3.2比25.2±6.9,均P<0.01],IL-10含量(ng/g)顯著升高(47.3±5.5比27.6±10.2,P<0.01),HO-1錶達明顯增加.Glu+ZnPP組與模型組各指標比較差異無統計學意義.結論 Glu能明顯增彊內毒素血癥大鼠腸組織HO-1錶達,明顯減輕腸道炎癥反應,從而保護腸黏膜.
목적 탐토곡안선알(Glu)대내독소혈증대서장도손상적보호작용이급대혈홍소가양매-1(HO-1)표체적영향.방법 안수궤수자표법장32지웅성SD 대서분위정상대조조、모형조、Glu조화Glu+자원계람(ZnPP)조,매조8지.복강주사내독소지다당(LPS)10 mg/kg제비내독소혈증동물모형.Glu조주사LPS전12 h관위Glu 1 g/kg;Glu+ZnPP조주사LPS전12 h관위Glu 1 g/kg,주사LPS전1 h정맥주사ZnPP 10 mmol/kg.술후12 h취회장조직,측정수과양화물매(MPO)활성급종류배사인자-α(TNF-α)、백세포개소-10(IL-10)함량,병진행장조직학평분;용면역조화법검측회장조직HO-1표체.결과 여정상대조조비교,모형조회장조직학평분、MPO활성급TNF-α、IL-10함량현저승고[조직학평분(분):3.3±0.4비1.1±0.6,MPO활성(U/g):0.40±0.08비0.26±0.07,TNF-α함량(ng/g):25.2±6.9비6.5±2.8,IL-10함량(ng/g):27.6±10.2비5.7±2.9,균P<0.01];HO-1표체교저.여모형조비교,Glu조조직학평분、MPO활성화TNF-α함량명현감저[조직학평분(분):1.6±0.5비3.3±0.4,MPO활성(U/g):0.25±0.05비0.40±0.08,TNF-α함량(ng/g):13.4±3.2비25.2±6.9,균P<0.01],IL-10함량(ng/g)현저승고(47.3±5.5비27.6±10.2,P<0.01),HO-1표체명현증가.Glu+ZnPP조여모형조각지표비교차이무통계학의의.결론 Glu능명현증강내독소혈증대서장조직HO-1표체,명현감경장도염증반응,종이보호장점막.
Objective To investigate the protective effect of glutamine (Glu) pretreatment on intestinal injury induced by endotoxin and expression of heme oxygenase-1 (HO-1) in rats.Methods Thirty-two male Sprague-Dawley (SD) rats were randomly divided into four groups (n=8 in each group): normal control group, model group, Glu group and Glu+zinc protoporphyrin (ZnPP) group. In model group, endotoxemia was produced by intraperitoneal injection of lipopolysaccharide (LPS, 10 mg/kg). In Glu group, the rats received intragastraiclly 1 g/kg of Glu 12 hours before LPS intraperitoneal injection. In Glu+ZnPP group, the rats received 1 g/kg of Glu by gavage 12 hours before LPS intraperitoneal injection and ZnPP 10 mmol/kg intravenously via tail vein 1 hour before LPS injection. The distal ileum was harvested in full thickness 12 hours after LPS injection. The myeloperoxidase (MPO) activity, tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in the intestine were determined, the pathologic changes were observed and expressed in Chiu grade. The expression of HO-1 was evaluated by immunohistochemistry method. Results Compared with normal control group, the Chiu grade, MPO activity, the content of TNF-α and IL-10 were significantly increased in model group [Chiu grade: 3.3±0.4 vs. 1.1±0.6, MPO activity (U/g): 0.40±0.08 vs. 0.26±0.07, TNF-α (ng/g): 25.2±6.9 vs. 6.5±2.8, IL-10 (ng/g): 27.6±10.2 vs. 5.7±2.9, all P<0.01], and the expression of HO-1 was decreased. Compared with model group, the Chiu grade, MPO activity, the content of TNF-α in Glu group were significantly decreased [Chiu grade: 1.6±0.5 vs. 3.3±0.4, MPO activity (U/g): 0.25±0.05 vs. 0.40±0.08, the content of TNF-α (ng/g): 13.4±3.2 vs. 25.2±6.9, all P<0.01], while the level of IL-10 (ng/g) elevated (47.3±5.5 vs. 27.6±10.2, P<0.01), and the expression of HO-1 was increased. There was no difference in above mentioned indexes between model group and Glu+ZnPP group. Conclusion Glu pretreatment significantly ameliorates the expression of HO-1 of intestinal tissue induced by LPS in rats, and intestinal mucosa is protected with alleviation of inflammatory reaction in intestinal tract.
