CAJ | 학술논문

目的探讨鞘内注射艾芬地尔对骨癌痛小鼠脊髓N-甲基-D-天冬氨酸(NMDA)受体2B亚基(NR2B)mRNA表达的影响.方法雄性C3H/HeJ小鼠140只,体重20～25 g,4～6周龄,随机分为5组(n=28):假手术组(S组)、骨癌病组(B组)和艾芬地尔2.5μg、5μg、10μg组(I1-3组).I1-3组和B组于小鼠右侧股骨远端骨髓腔接种NCTC 2472溶骨性纤维肉瘤细胞,建立骨癌痛模型;S组不接种肿瘤细胞.I1～3组于接种肿瘤细胞后14 d分别鞘内注射艾芬地尔2.5、5、10 μg,B组和S组鞘内注射艾芬地尔溶媒.各组于接种肿瘤细胞前1 d、鞘内注射艾芬地尔或溶媒前1 h、注射后2、12和24 h(T1～5)时随机取7只小鼠测定机械痛阈和热痛阈,并于T2～5,时测定后断头处死,取L3～5脊髓,采用RT-PCR法测定脊髓组织NR2B mRNA的表达水平.结果与S组比较,除I3,组T3时热痛阈差异无统汁学意义(P0.05)外,B组和I1～3组机械痛阈和热痛阈均降低(P<0.05),B组和I1组脊髓组织NR2B mRNA表达上调,I2组该指标表达下调(P<0.05);与B组比较,I2.3组机械痈阈和热痛阈升高,脊髓组织NR2B mRNA表达下调(P<0.05),I1组各时点以上指标差异均无统计学意义(P0.05);与I2组比较,I3组机械痛阈和热痛阈升高,脊髓组织NR2B mRNA表达下调(P<0.05).结论鞘内注射艾芬地尔可通过阻断含2B亚基的NMDA受体缓解小鼠骨癌痛,并下凋脊髓组织NR2B mRNA的表达抑制痛敏反应.
목적 탐토초내주사애분지이대골암통소서척수N-갑기-D-천동안산(NMDA)수체2B아기(NR2B)mRNA표체적영향.방법 웅성C3H/HeJ소서140지,체중20～25 g,4～6주령,수궤분위5조(n=28):가수술조(S조)、골암병조(B조)화애분지이2.5μg、5μg、10μg조(I1-3조).I1-3조화B조우소서우측고골원단골수강접충NCTC 2472용골성섬유육류세포,건립골암통모형;S조불접충종류세포.I1～3조우접충종류세포후14 d분별초내주사애분지이2.5、5、10 μg,B조화S조초내주사애분지이용매.각조우접충종류세포전1 d、초내주사애분지이혹용매전1 h、주사후2、12화24 h(T1～5)시수궤취7지소서측정궤계통역화열통역,병우T2～5,시측정후단두처사,취L3～5척수,채용RT-PCR법측정척수조직NR2B mRNA적표체수평.결과 여S조비교,제I3,조T3시열통역차이무통즙학의의(P0.05)외,B조화I1～3조궤계통역화열통역균강저(P<0.05),B조화I1조척수조직NR2B mRNA표체상조,I2조해지표표체하조(P<0.05);여B조비교,I2.3조궤계옹역화열통역승고,척수조직NR2B mRNA표체하조(P<0.05),I1조각시점이상지표차이균무통계학의의(P0.05);여I2조비교,I3조궤계통역화열통역승고,척수조직NR2B mRNA표체하조(P<0.05).결론 초내주사애분지이가통과조단함2B아기적NMDA수체완해소서골암통,병하조척수조직NR2B mRNA적표체억제통민반응.
Objective To investigate the effect of intrathecal injection of ifenprodil on the expression of 2B subunit mRNA of NMDA receptor (NR2B) in spinal cord in a mouse model of bone cancer pain. Methods One hundred and forty male C3H/HeJ mice weighing 20-25 g were randomly divided into 5 groups (n=28 each):group S sham operation; group B bone cancer pain; group I1- ifenprodil 2.5, 5 and 10 μg. Bone cancer pain was performed by intra-right-femur inoculations of osteolytic NCTC 2472 cells in group I1- and B. On the 14 d after inoculations, group I1-3 received intrathecal injection of ifenprodil 2.5, 5 and 10 μg dissolved in 5 μl solvent respectively, while group B and S received intrathecal injection of solvent. Seven mice was selected randomly from each group at 1 d before inoculations, at 1 h before, intrathecal injection of ifenprodil or solvent and at 2, 12 and 24 h after the injection (T1-5) tor determination of mechanical and thermal pain threshold. The mice were decapitated at T2.5 after determination of pain threshold and the L3-5 part of spinal cord was removed for determination of the expression of NR2B mRNA by RT-PCR. Results The mechanical and thermal pain threshold were significantly lower in group B and I1-3(P<0.05), except for the thermal pain threshold at T3 in group I3(P>0.05), and the expression of NR2B mRNA was significantly higher in group B and I3 while lower in group I2.3 than in group S (P<0.05). The mechanical and thermal pain threshold were significantly higher and the expression of NR2B mRNA was lower in group I2.3 than in group B (P<0.05), but there were no significant differences in the indexes mentioned above between group I1 and B (P>0.05). The mechanical and thermal pain threshold were significantly higher and the expression of NR2B mRNA was lower in group I3 than in group I2 (P <0.05). Conclusion lntratheeal injection of ifenprodil can attenuate bone cancer pain through blocking NR2B and inhibit hyperalgesia through down-regulating the NR2B mRNA expression in spinal cord.