CAJ | 학술논문

目的观察大鼠部分肝缺血再灌注损伤后切除对残肝再生的影响.方法将75只健康雄性SD大鼠随机分为5组:肝脏左叶和中叶(约占全肝70%)切除组(Control组)、肝脏左叶和中叶缺血10min再灌注30min后切除组(I10R30组)、类推得到I60R30组、I90R30组、I90R60组.术后6、12、24h等时间点,测定再生肝重量(RLW);自动生化分析仪检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)含量;酶联免疫吸附试验(ELISA)检测血清肿瘤坏死因子(TNF)-α含量;通过免疫组织化学法检测残肝增殖细胞核抗原(Ki-67)表达.结果术后12h,I60R30、I90R30和I90R60组RLW值分别为(1.80±0.03)%、(1.82±0.10)%、(1.87±0.05)%;Ki-67值分别为(58.35±2.18)%、(59.73±3.06)%、(62.65±2.24)%,均明显高于对照组(P<0.05).缺血再灌注干预各组ALT和AST明显高于对照组(P<0.05).术后6h和12h,I60R30、I90R30和I90R60组TNF-α明显高于对照组(P<0.05).结论大鼠即将被切除的肝脏先缺血再灌注后切除,对残肝再生具有促进作用;诱导产生的TNF-α表达量增多是促进肝再生的原因之一.
목적 관찰대서부분간결혈재관주손상후절제대잔간재생적영향.방법 장75지건강웅성SD대서수궤분위5조:간장좌협화중협(약점전간70%)절제조(Control조)、간장좌협화중협결혈10min재관주30min후절제조(I10R30조)、유추득도I60R30조、I90R30조、I90R60조.술후6、12、24h등시간점,측정재생간중량(RLW);자동생화분석의검측혈청병안산전안매(ALT)、천동안산전안매(AST)함량;매련면역흡부시험(ELISA)검측혈청종류배사인자(TNF)-α함량;통과면역조직화학법검측잔간증식세포핵항원(Ki-67)표체.결과 술후12h,I60R30、I90R30화I90R60조RLW치분별위(1.80±0.03)%、(1.82±0.10)%、(1.87±0.05)%;Ki-67치분별위(58.35±2.18)%、(59.73±3.06)%、(62.65±2.24)%,균명현고우대조조(P<0.05).결혈재관주간예각조ALT화AST명현고우대조조(P<0.05).술후6h화12h,I60R30、I90R30화I90R60조TNF-α명현고우대조조(P<0.05).결론 대서즉장피절제적간장선결혈재관주후절제,대잔간재생구유촉진작용;유도산생적TNF-α표체량증다시촉진간재생적원인지일.
Objective To investigate the effects of ischemia reperfusion injury before partial hepatectomy on liver regeneration in rats. Methods Seventy-five male healthy SD rats were randomly classified into 5 groups: group control, in which rats were only subjected to 70% hepatectomy; group I10R30, 70% liver hepatectomy after 10 min of ischemia and 30 min of reperfusion in the resected liver; By analogy, group I60R30, group I90R30 and group I90R60 were constructed. At 6th, 12th and 24th h after operation, RLW was determined; serum alanine aminotransferase (ALT) and aspartate transaminase (AST) activities were measured by using autoanalyzer; the levels of serum tumor necrosis factor (TNF)-α were determined by ELISA and the expression level of Ki-67 was detected by using immunohistochemical methods in the residual liver tissues. Results At 12th h after partial hepatectomy, the rate of RLW in group I60R30, group I90R30 and group I90R60 was (1.80±0.03)%, (1.82±0.10)%, (1.87±0.05)% respectively; the rate of Ki-67 was (58.35±2.18)%, (59.73±3.06)%, (62.65±2.24)% respectively, which was significantly higher than that in the group control (P<0.05). The levels of ALT and AST in rats with ischemia reperfusion injury were higher than in the group control (P<0.05). At 6th h and 12th h after operation, the expression levels of TNF-α in groups I60R30, I90R30 and I90R60 were significantly higher than those in the group control (P<0.05). Conclusion Ischemia reperfusion injury in the resected liver before partial hepatectomy could improve liver regeneration of the remnant liver in rats. The high expression of induced TNF-α may be one of the reasons.