CAJ | 학술논문

利用发状念珠藻作为实验材料，研究了温度对其吸水、光合活性恢复和抗氧化活性的影响．研究结果显示，发状念珠藻的吸水量和对水的亲和力在30℃时最强，光合活性在25—30℃恢复较好，高温和低温对其光合活性恢复均有明显的抑制作用．经过不同冷冻时间处理后电解质渗透率、可溶性糖、游离脯氨酸和丙二醛含量在短期内均上升：过氧化氢酶活性下降；超氧化物歧化酶活性先上升后下降．发状念珠藻吸水和光合活性恢复的最适温度大约在30℃，在短期内可以通过调整抗氧化活性，使其对低温具有一定的耐受力．
이용발상념주조작위실험재료，연구료온도대기흡수、광합활성회복화항양화활성적영향．연구결과현시，발상념주조적흡수량화대수적친화력재30℃시최강，광합활성재25—30℃회복교호，고온화저온대기광합활성회복균유명현적억제작용．경과불동냉동시간처리후전해질삼투솔、가용성당、유리포안산화병이철함량재단기내균상승：과양화경매활성하강；초양화물기화매활성선상승후하강．발상념주조흡수화광합활성회복적최괄온도대약재30℃，재단기내가이통과조정항양화활성，사기대저온구유일정적내수력．
In this paper, effects of temperature on absorbing water, recovery of photosynthetic activity and an- tioxidant activity in N. flagelliforme were investigated. The results showed that there are the strongest affinity for water and the greatest capacity of absorbing water at 30℃. The chlorophyll a fluorescence parameters were measured by saturated pulse, the maximal photochemical efficieng of PSII photochemistry （ Fv/Fm ）, actual photochemical efficiency of PSII （Yield） at 25 - 30℃ could maintain a higher level with the culture time, whereas the high temperature and low temperature resulted in a significant decrease. The water-saturated alga was exposed to - 18℃ for different time. Electrolyte leakage （ EL）, contents of soluble sugar （ WSS）, free proline （Fpro） and malondialdehyde （MDA）, superoxide dismutase （SOD） and catalase （CAT） activity, were determined. The results showed that EL, contents of WSS, Fpro and MDA significantly increased, however, Chla content and CAT activity significantly decreased with the exposed time. SOD activity rose at the beginning and then reduced with the extension of freezing. The above results indicated that the optimum temperature of the wa- ter-absorption and photosynthetic recovery in N. flagelliform is at approximately 30 ℃, and this alga has certain tolerance to low temperature circumstances through antioxidant mechanism in short time.