CAJ | 학술논문

目的通过研究病变宫颈中人乳头状瘤病毒(HPV)16/18型的表达,探讨HPV16/18型病毒感染与宫颈病变发生发展之间的关系.方法结合病理切片诊断,以免疫组化作对照.运用实时荧光定量PCR技术检测病变宫颈中HPV16/18型DNA拷贝数,以及HPV16/18型E7基因mRNA表达量.结果慢性宫颈炎患者中HPV16/18型感染率低(7.4%).宫颈管上皮内瘤样变(CIN)组HPV16型感染率较高为69.6%,宫颈癌患者巾为72.7%.HPV16型DNA的拷贝数在宫颈上皮内瘤样变患者中与病理分级没有明显的相关性.但在宫颈癌患者中,病毒DNA的拷贝数明显升高,二者差异明显.CIN轻度(I)、中度(Ⅱ)、高度(Ⅲ)组和宫颈癌患者中,HPV16 E7基冈的表达率分别为0、37.5%、42.9%、63.6%.统计学分析表明,HPV16 E7 mRNA的拷贝数与病情呈明显的正相关性.结论感染者中主要以HPV16型为主,HPV18型较少.宫颈癌患者中HPV16 DNA拷贝数明显高于CIN Ⅱ、Ⅲ组,HPV16型E7 mRNA在宫颈癌中表达率及表达量明显增加并与宫颈癌变呈正相关.实时荧光定量PCR适合临床宫颈病变病毒的筛查与检测.
목적 통과연구병변궁경중인유두상류병독(HPV)16/18형적표체,탐토HPV16/18형병독감염여궁경병변발생발전지간적관계.방법 결합병리절편진단,이면역조화작대조.운용실시형광정량PCR기술검측병변궁경중HPV16/18형DNA고패수,이급HPV16/18형E7기인mRNA표체량.결과 만성궁경염환자중HPV16/18형감염솔저(7.4%).궁경관상피내류양변(CIN)조HPV16형감염솔교고위69.6%,궁경암환자건위72.7%.HPV16형DNA적고패수재궁경상피내류양변환자중여병리분급몰유명현적상관성.단재궁경암환자중,병독DNA적고패수명현승고,이자차이명현.CIN경도(I)、중도(Ⅱ)、고도(Ⅲ)조화궁경암환자중,HPV16 E7기강적표체솔분별위0、37.5%、42.9%、63.6%.통계학분석표명,HPV16 E7 mRNA적고패수여병정정명현적정상관성.결론 감염자중주요이HPV16형위주,HPV18형교소.궁경암환자중HPV16 DNA고패수명현고우CIN Ⅱ、Ⅲ조,HPV16형E7 mRNA재궁경암중표체솔급표체량명현증가병여궁경암변정정상관.실시형광정량PCR괄합림상궁경병변병독적사사여검측.
Objective To study the relation between the HPV6/18 virus infection and the development of pathological changes of cervix. Methods The number of HPV16/18 DNA copies and the expression rate of HPV16/18 E7 mRNA in the pathological cervix were examined by the quantitative fluorescent PCR combined with pathological diagnosis and immunohistochemistry staining. Results The HPV16 infection rates in chronic cervicitis group were much lower (7.4%) than that in the cervical intraepithelial neoplasia (CIN) groups and the cervical cancer group (69.6% and 72.7%), respectively. Statistical analysis showed that the difference of HPV16 DNA copies was not significant between the chronic cervicitis group and CIN groups. In contrast to the above mentioned result, the number of HPV DNA copies between the CIN groups and the cervical cancer group was significantly different. The HPV16 E7 gene expression rates in CIN Ⅰ, Ⅱ, Ⅲ and cervical cancer groups were 0,37.5%,42.9%,63.6%, respectively. Conclusion Ins more common than that with HPV18. The number of HPV16 DNA copies in cervical cancer tissues is markedly higher than that in CIN Ⅱ, Ⅲ groups. The HPV16 E7 mRNA expression is significantly increased in the cervical cancer, and it is more closely correlated to this pathological changes. The quantitative fluorescent PCR can be used to reflect the activity of HPV, and it is a useful method for the screening examination of HPV and for the early diagnosis and treatment of cervical caner.