中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
6期
406-410
,共5页
陈星星%晏丹%尤春景%袁修学%刘亚昆%王熠钊%向艳平%秦开蓉%徐江
陳星星%晏丹%尤春景%袁脩學%劉亞昆%王熠釗%嚮豔平%秦開蓉%徐江
진성성%안단%우춘경%원수학%류아곤%왕습쇠%향염평%진개용%서강
脊髓损伤%神经干细胞%高压氧
脊髓損傷%神經榦細胞%高壓氧
척수손상%신경간세포%고압양
Spinal cord injury%Neural stem cells%Hyperbaric oxygen
目的 观察神经干细胞(NSCs)移植联合高压氧(HBO)治疗对脊髓损伤(SCI)大鼠功能恢复的影响.方法 共选取40只成年健康SD大鼠,采用随机数字表法将其分为假手术组、脊髓损伤组(SCI组)、神经干细胞移植组(NSCs组)及高压氧联合神经干细胞移植组(HBO+NSCs组).将上述各组大鼠制成脊髓全横断损伤模型,NSCs组及HBO+NSCs组于术后分别给予NSCs移植治疗,HBO+NSCs组大鼠在此基础上辅以HBO治疗.于制模后不同时间点采用斜板实验、BBB评分对各组大鼠进行运动功能评定,同时采用运动诱发电位(MEP)评估各组大鼠神经传导功能,并观察各组大鼠脊髓损伤处移植NSCs存活情况.结果 从脊髓损伤后第2周开始,HBO+NSCs组大鼠斜板实验和BBB评分结果均显著优于SCI组及NSCs组(P＜0.05).在脊髓损伤后第4周时,SCI组仍无法引出MEP,而HBO+NSCs组及NSCs组MEP波形均有所恢复,并且HBO+NSCs组潜伏期较NSCs组显著缩短(P＜0.05)；同时HBO+NSCs组受损脊髓存活的NSCs数量显著多于NSCs组(P＜0.05).结论 HBO联合NSCs移植治疗SCI大鼠具有协同效应,能进一步促进SCI大鼠肢体运动功能及神经传导功能恢复,其治疗机制可能与HBO促进大鼠脊髓损伤处移植NSCs存活有关.
目的 觀察神經榦細胞(NSCs)移植聯閤高壓氧(HBO)治療對脊髓損傷(SCI)大鼠功能恢複的影響.方法 共選取40隻成年健康SD大鼠,採用隨機數字錶法將其分為假手術組、脊髓損傷組(SCI組)、神經榦細胞移植組(NSCs組)及高壓氧聯閤神經榦細胞移植組(HBO+NSCs組).將上述各組大鼠製成脊髓全橫斷損傷模型,NSCs組及HBO+NSCs組于術後分彆給予NSCs移植治療,HBO+NSCs組大鼠在此基礎上輔以HBO治療.于製模後不同時間點採用斜闆實驗、BBB評分對各組大鼠進行運動功能評定,同時採用運動誘髮電位(MEP)評估各組大鼠神經傳導功能,併觀察各組大鼠脊髓損傷處移植NSCs存活情況.結果 從脊髓損傷後第2週開始,HBO+NSCs組大鼠斜闆實驗和BBB評分結果均顯著優于SCI組及NSCs組(P＜0.05).在脊髓損傷後第4週時,SCI組仍無法引齣MEP,而HBO+NSCs組及NSCs組MEP波形均有所恢複,併且HBO+NSCs組潛伏期較NSCs組顯著縮短(P＜0.05)；同時HBO+NSCs組受損脊髓存活的NSCs數量顯著多于NSCs組(P＜0.05).結論 HBO聯閤NSCs移植治療SCI大鼠具有協同效應,能進一步促進SCI大鼠肢體運動功能及神經傳導功能恢複,其治療機製可能與HBO促進大鼠脊髓損傷處移植NSCs存活有關.
목적 관찰신경간세포(NSCs)이식연합고압양(HBO)치료대척수손상(SCI)대서공능회복적영향.방법 공선취40지성년건강SD대서,채용수궤수자표법장기분위가수술조、척수손상조(SCI조)、신경간세포이식조(NSCs조)급고압양연합신경간세포이식조(HBO+NSCs조).장상술각조대서제성척수전횡단손상모형,NSCs조급HBO+NSCs조우술후분별급여NSCs이식치료,HBO+NSCs조대서재차기출상보이HBO치료.우제모후불동시간점채용사판실험、BBB평분대각조대서진행운동공능평정,동시채용운동유발전위(MEP)평고각조대서신경전도공능,병관찰각조대서척수손상처이식NSCs존활정황.결과 종척수손상후제2주개시,HBO+NSCs조대서사판실험화BBB평분결과균현저우우SCI조급NSCs조(P＜0.05).재척수손상후제4주시,SCI조잉무법인출MEP,이HBO+NSCs조급NSCs조MEP파형균유소회복,병차HBO+NSCs조잠복기교NSCs조현저축단(P＜0.05)；동시HBO+NSCs조수손척수존활적NSCs수량현저다우NSCs조(P＜0.05).결론 HBO연합NSCs이식치료SCI대서구유협동효응,능진일보촉진SCI대서지체운동공능급신경전도공능회복,기치료궤제가능여HBO촉진대서척수손상처이식NSCs존활유관.
Objective To investigate the effects of hyperbaric oxygen (HBO) combined with neural stem cell (NSC) transplantation on functional recovery after spinal cord injury (SCI).Methods Forty healthy adult Sprague-Dawley rats were randomly divided into a sham group,an SCI group,an NSC group and an HBO+NSC group.In the latter three groups,complete spinal cord transection was performed at T10.The rats of the HBO+NSC group were treated with HBO therapy after NSC transplantation.Hindlimb motor function was assessed with the BassoBeattie-Bresnahan (BBB) scale and tilt table testing.Motor evoked potentials (MEPs) were measured to evaluate the rats'nerve conduction function.The amount of surviving transplanted NSCs at the injured site was counted using Hoechst dyeing.Results The average BBB scores and tilt table test results in the HBO+NSCs group were significantly better than in the SCI and NSC groups from the 2nd week post-SCI.By the 4th week the average latency period and MEP amplitude in the HBO+NSC group were significantly better than in the NSC group,while MEPs in the SCI group still had not re-appeared.The surviving amount of transplanted NSCs in the HBO+NSC group was also significantly greater than in the NSC group.Conclusion HBO combined with NSC transplantation has synergistic effects and can improve functional recovery after SCI.This may be explained by the important role of HBO in promoting the survival of transplanted NSCs.
