CAJ | 학술논문

目的观察雌激素对大鼠全层皮肤缺损创面愈合过程中微小血管再形成的影响.方法将40只雌性Wistar大鼠随机分成3组:单纯损伤组15只(A组),去卵巢组15只(B组)和假手术组10只(C组).8周后,在背部制成全层全损皮肤创伤愈合模型,分别于伤后1、3、7、14和21 d记录3组各个时间点伤腔容积,并在创缘取材.常规组织学观察皮肤愈合过程和微血管的数量,同时采用雌激素β受体(ER-β)进行免疫组织化学染色,观察微血管及周围细胞受体的染色.结果　比较各个时间点的伤腔容积,单纯损伤组和假手术组大鼠创面内肉芽组织形成量明显大于去卵巢组,组织学评分略高于去卵巢大鼠;而单纯损伤组和假手术组之间差异无统计学意义(P＞0.05).卵巢切除大鼠伤后各时间点,真皮内微小血管的数量较单纯损伤组减少,各组均于伤后7 d,随着成纤维细胞和毛细血管数目的增加,肉芽组织生长达到高峰.同时,各损伤组创基内微小血管周围均出现较为幼稚的细胞,其表面可以表达ER-β,但卵巢切除组(159.91±12.65)稍弱于单纯损伤组(189.36±27.32),差异无统计学意义(P＞0.05).结论　大鼠去卵巢后创面愈合速度明显减慢与微血管形成有关,雌激素及其受体在该过程中有重要作用.
목적 관찰자격소대대서전층피부결손창면유합과정중미소혈관재형성적영향.방법 장40지자성Wistar대서수궤분성3조:단순손상조15지(A조),거란소조15지(B조)화가수술조10지(C조).8주후,재배부제성전층전손피부창상유합모형,분별우상후1、3、7、14화21 d기록3조각개시간점상강용적,병재창연취재.상규조직학관찰피부유합과정화미혈관적수량,동시채용자격소β수체(ER-β)진행면역조직화학염색,관찰미혈관급주위세포수체적염색.결과　비교각개시간점적상강용적,단순손상조화가수술조대서창면내육아조직형성량명현대우거란소조,조직학평분략고우거란소대서;이단순손상조화가수술조지간차이무통계학의의(P＞0.05).란소절제대서상후각시간점,진피내미소혈관적수량교단순손상조감소,각조균우상후7 d,수착성섬유세포화모세혈관수목적 증가,육아조직생장체도고봉.동시,각손상조창기내미소혈관주위균출현교위유치적세포,기표면가이표체ER-β,단란소절제조(159.91±12.65)초약우단순손상조(189.36±27.32),차이무통계학의의(P＞0.05).결론　대서거란소후창면유합속도명현감만여미혈관형성유관,자격소급기수체재해과정중유중요작용.
Objective To investigate the effect of estrogen on the revascularization of full-thickness skin wounds healing in rats. Methods Forty 2-month-old female Wistar rats were divided into three groups: control group (group A, pure injury,n = 15), ovariectomized group (group B,n = 15) and sham operation group (group C,n = 10 ). After 8 weeks, full-thickness wounds model, 18 mm in diameter, were made on the back of the each animal. The volume of wound was measured at each time point. The specimens of wound edge were obtained at the 1st, 3rd, 7th, 14th and 21st day after injury. The process of wound healing and the number of microvessels in the granulation tissue were evaluated by Haematine-Eosin (HE) staining. The immunohistochemical staining was used to detect the expression of estrogen receptor β (ER-β) in microvascular endothelial cells and their surrounding cells. Results To compare the volume of wound at each time point, the granulation tissue formation in group A and group C was significantly higher than that in group B, but there was no significant difference between group A and group C. The number of capillary blood vessels in group B was significantly reduced as compared with that in group A. The expression of ER-β was positive on the cells surface in all groups. ER-β expression in group B was weaker than in group A ( 159. 91 ± 12. 65 v. s 189. 36 ±27. 32,P ＜0. 05). Conclusion The delay of wound healing in ovariectomized rats has a great relationship with angiogenesis. Estrogen and its receptor might play important roles in the process.