CAJ | 학술논문

目的研究人疱疹病毒7型(HHV-7)病毒感染与噬血细胞综合征发生的关系,为临床噬血细胞综合征患者诊断和抗病毒的治疗提供依据.方法收集噬血细胞综合征患者不同治疗期外周血,提取DNA,合成HHV-7引物,PCR扩增片段,基因序列分析,荧光定量PCR法测定病毒载量,化学发光法测定血液铁蛋白的浓度,分析二者的相关性.结果经PCR扩增和基因序列分析在噬血细胞综合征外周血中检测到HHV-7基因片段,其HHV-7病毒载量随着血液铁蛋白的浓度而变化.结论 HHV-7感染可以继发引起噬血细胞综合征并且与HHV-7病毒载量有关.
목적 연구인포진병독7형(HHV-7)병독감염여서혈세포종합정발생적관계,위림상서혈세포종합정환자진단화항병독적치료제공의거.방법 수집서혈세포종합정환자불동치료기외주혈,제취DNA,합성HHV-7인물,PCR확증편단,기인서렬분석,형광정량PCR법측정병독재량,화학발광법측정혈액철단백적농도,분석이자적상관성.결과 경PCR확증화기인서렬분석재서혈세포종합정외주혈중검측도HHV-7기인편단,기HHV-7병독재량수착혈액철단백적농도이변화.결론 HHV-7감염가이계발인기서혈세포종합정병차여HHV-7병독재량유관.
Objective To research the relationship between human herpesvirus 7 (HHV-7) viral Load and the etiopathogenisis of hemophagocytic syndrome, in order to provide evidence for the clinical diagnosis of hemophagocytic syndrome and anti-virus therapy. Methods Peripheral blood of patient with hemophagocytic syndrome during different treatment periods, extracted DNA, Syntheticed the primers of HHV-7,gene sequence of PCR amplified fragments detected, determined HHV-7 viral Load by Real - time fluorescent quantitative PCR and the ferritin concentration in peripheral blood detected by chemiluminescence. Result The sequence result indicated that PCR amplified fragment was a part of HHV-7 gene, the ferritin concentration viried with the load of HHV-7. Conclusion The occurrence of hemophagocytic syndrome is connetted with the load of HHV-7.