中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
CHINESE JOURNAL OF GENERAL SURGERY
2012年
10期
812-815
,共4页
杨成广%郑颖%邱文才%常新
楊成廣%鄭穎%邱文纔%常新
양성엄%정영%구문재%상신
动物实验%受体,胃促生长素%肠肌丛
動物實驗%受體,胃促生長素%腸肌叢
동물실험%수체,위촉생장소%장기총
Animal experimentation%Receptors,Ghrelin%Myenteric plexus
目的 探讨Ghrelin对大鼠小肠平滑肌舒缩活动的影响和机制.方法 观察不同浓度Ghrelin(0、20、40、80 μg/kg)对迷走神经切断大鼠小肠转运的影响,Ghrelin(0.01、0.1、0.5、1.0 μmol/L)对体外大鼠小肠平滑肌肌条舒缩活动的影响,免疫荧光方法检测Ghrelin受体(GHSR1a)在小肠肌层中不同类型细胞的分布.结果 Ghrelin在外周剂量依赖性地增加小肠转运率[(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％、(44.7±2.1)％],增强卡巴胆碱引起的肌条收缩[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]和舒张[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]效应,Ghrelin受体主要分布在肠内肌层中的神经细胞膜上,平滑肌细胞膜上无分布.结论 Ghrelin可通过直接作用于小肠肌层神经丛中的神经细胞而增强胆碱能神经递质引起的小肠平滑肌的舒缩活动.
目的 探討Ghrelin對大鼠小腸平滑肌舒縮活動的影響和機製.方法 觀察不同濃度Ghrelin(0、20、40、80 μg/kg)對迷走神經切斷大鼠小腸轉運的影響,Ghrelin(0.01、0.1、0.5、1.0 μmol/L)對體外大鼠小腸平滑肌肌條舒縮活動的影響,免疫熒光方法檢測Ghrelin受體(GHSR1a)在小腸肌層中不同類型細胞的分佈.結果 Ghrelin在外週劑量依賴性地增加小腸轉運率[(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％、(44.7±2.1)％],增彊卡巴膽堿引起的肌條收縮[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]和舒張[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]效應,Ghrelin受體主要分佈在腸內肌層中的神經細胞膜上,平滑肌細胞膜上無分佈.結論 Ghrelin可通過直接作用于小腸肌層神經叢中的神經細胞而增彊膽堿能神經遞質引起的小腸平滑肌的舒縮活動.
목적 탐토Ghrelin대대서소장평활기서축활동적영향화궤제.방법 관찰불동농도Ghrelin(0、20、40、80 μg/kg)대미주신경절단대서소장전운적영향,Ghrelin(0.01、0.1、0.5、1.0 μmol/L)대체외대서소장평활기기조서축활동적영향,면역형광방법검측Ghrelin수체(GHSR1a)재소장기층중불동류형세포적분포.결과 Ghrelin재외주제량의뢰성지증가소장전운솔[(25.4±1.0)％、(33.7±1.9)％、(39.3±2.4)％、(44.7±2.1)％],증강잡파담감인기적기조수축[(67.0±2.4)％、(149.5±3.3)％、(187.1±4.7)％、(213.5±3.4)％]화서장[(35.3±1.1)％、(62.9±3.8)％、(79.6±2.7)％、(94.6±2.2)％]효응,Ghrelin수체주요분포재장내기층중적신경세포막상,평활기세포막상무분포.결론 Ghrelin가통과직접작용우소장기층신경총중적신경세포이증강담감능신경체질인기적소장평활기적서축활동.
Objective To investigate the effect and mechanism of Ghrelin on the contraction and relaxation of rat small intestinal smooth muscle. Methods The effect of different concentrations of Ghrelin (0;20;40;80 μg/kg) on the small intestinal transit in vagotomized rats in vivo and Ghrelin (0.01; 0.1;0.5 ; 1.0 μmol/L) on the contraction and relaxation of rat small intestinal smooth muscle strips in vitro was observed,the locations of Ghrelin receptors (GHS-R1 a) in small intestinal muscle layers were detected by immunofluorescency. Results Ghrelin dose-dependently increases small intestinal transit (( 25.4 ± 1.0)％,(33.7 ± 1.9)％,(39.3 ±2.4)％,(44.7 ±2.1)％),enhances the contraction ((67.0 ±2.4)％,(149.5 ±3.3)％,(187.1 ±4.7)％,(213.5 ±3.4)％) and relaxation ((35.3 ± 1.1)％,(62.9 ± 3.8 ) ％,( 79.6 ± 2.7 ) ％,( 94.6 ± 2.2 ) ％ ) of smooth muscle strips mediated by carbachol.Ghrelin receptors were mainly located on membrane of the nerve cells in the muscle layers,while no receptors exist on membrane of smooth muscle cells. Conclusions Ghrelin enhances the effect of contraction and relaxation of rat small intestinal smooth muscle mediated by cholinergic neurotransmitters activating nerve cells in the enteric plexus.
