CAJ | 학술논문

目的观察串珠素在大鼠椎板切除术后硬膜外瘢痕形成过程中的表达.方法 60只Wistar大鼠随机分为五组:1周组、2周组、4周组、6周组和对照组.实验组(1周组、2周组、4周组及6周组)大鼠制作腰椎椎板切除模型,分别于术后1周、2周、4周或6周行二次手术取硬膜外瘢痕；对照组大鼠取椎板后肌肉组织.行HE染色观察实验组硬膜外瘢痕组织的形成情况；行免疫组织化学检测(Envision法)观察串珠素在硬膜外瘢痕组织中的细胞来源；以Western blot法和RT-PCR法检测硬膜外瘢痕组织中串珠素表达量的变化.结果 HE染色显示随观察时间延长瘢痕组织逐渐形成.免疫组织化学检测结果显示串珠素在瘢痕组织中的成纤维细胞胞质中呈阳性表达.Western blot和RT-PCR结果显示1周组、2周组、4周组和6周组中串珠素有明显表达,与对照组比较差异有统计学意义(P＜0.01)；1周组、2周组和4周组的表达量逐渐增加,各组间差异有统计学意义(P＜0.01)；6周组和4周组比较,串珠素表达量减少,差异有统计学意义(P＜0.01).结论串珠素与硬膜外瘢痕组织的形成有关,硬膜外瘢痕组织中的成纤维细胞是串珠素的细胞来源.
목적 관찰천주소재대서추판절제술후경막외반흔형성과정중적표체.방법 60지Wistar대서수궤분위오조:1주조、2주조、4주조、6주조화대조조.실험조(1주조、2주조、4주조급6주조)대서제작요추추판절제모형,분별우술후1주、2주、4주혹6주행이차수술취경막외반흔；대조조대서취추판후기육조직.행HE염색관찰실험조경막외반흔조직적형성정황；행면역조직화학검측(Envision법)관찰천주소재경막외반흔조직중적세포래원；이Western blot법화RT-PCR법검측경막외반흔조직중천주소표체량적변화.결과 HE염색현시수관찰시간연장반흔조직축점형성.면역조직화학검측결과현시천주소재반흔조직중적성섬유세포포질중정양성표체.Western blot화RT-PCR결과현시1주조、2주조、4주조화6주조중천주소유명현표체,여대조조비교차이유통계학의의(P＜0.01)；1주조、2주조화4주조적표체량축점증가,각조간차이유통계학의의(P＜0.01)；6주조화4주조비교,천주소표체량감소,차이유통계학의의(P＜0.01).결론 천주소여경막외반흔조직적형성유관,경막외반흔조직중적성섬유세포시천주소적세포래원.
Objective To make a research on the expression of perlecan in the formation of epidural scar after posterior lumbar laminectomy in the rat.Methods Sixty Wistar rats were randomly and evenly divided into five groups:the experimental groups including A(the first week after surgery),B(the second week),C(the fourth week),D(the sixth week)and the control group.Laminectomy was performed on lumbar vertebra in each experimental group.The hematoxylin-eosin(HE)staining was used to observe the formation of epidural scar.Immunohistochemistry was performed to detect the perlecan located in the epidural scar.Western blot and RT-PCR were made to quantify the expression of the perlecan after surgery.Results According to HE,with prolonged,the scar tissue gradually formed.Immunohistochemistry demonstrated that the perlecan was expressed in the fibroblast.Western blot and RT-PCR showed the high level expression of perlecan in the experimental groups compared to the control group(P＜0.01).Furthermore,the expression of perlecan gradually increased with the prolongation of exposure time and had significantly statistical significance (P＜0.01)between the first three experimental groups.However,compared to the fourth week group,the expression of perlecan in the sixth week decreased obviously(P＜0.01).Conclusion Perlecan was expressed in the fibroblast of the epidural scar,and which plays an important role in the formation of epidural scar.