CAJ | 학술논문

目的探讨小鼠作业成绩与皮层中caveolin-1( Cav-1)蛋白表达的相关性.方法以昆明种小鼠为实验对象,建立y-迷宫模型,根据训练成绩将小鼠分为优良组与不良组,通过Western blot技术检测作业成绩不同的小鼠皮层、海马中Cav-1蛋白的表达并分析与作业成绩的相关性.结果作业成绩优良组小鼠皮层中Cav-1蛋白的表达(Cav-1/β-actin为5.71±1.11),高于作业成绩不良组(Cav-1/β-actin为1.69±0.20),差异具有显著性(P＜0.01)；而优良组小鼠海马中Car-1蛋白的表达(Cav-1/β-actin为0.22±0.09)与不良组(Cav-1/β-actin为0.26±0.04)相比,差异无显著性(P＞0.05).结论小鼠y-迷宫作业成绩优劣与脑内皮层中Cav-1蛋白的表达具有明显相关性.
목적 탐토소서작업성적여피층중caveolin-1( Cav-1)단백표체적상관성.방법 이곤명충소서위실험대상,건립y-미궁모형,근거훈련성적장소서분위우량조여불량조,통과Western blot기술검측작업성적불동적소서피층、해마중Cav-1단백적표체병분석여작업성적적상관성.결과 작업성적우량조소서피층중Cav-1단백적표체(Cav-1/β-actin위5.71±1.11),고우작업성적불량조(Cav-1/β-actin위1.69±0.20),차이구유현저성(P＜0.01)；이우량조소서해마중Car-1단백적표체(Cav-1/β-actin위0.22±0.09)여불량조(Cav-1/β-actin위0.26±0.04)상비,차이무현저성(P＞0.05).결론 소서y-미궁작업성적우렬여뇌내피층중Cav-1단백적표체구유명현상관성.
Objective To explore the relationship between operation scores and the expression of caveolin-1 ( Cav-1 ) protein in the cortex of mice.Methods Male Kunming mice were used and divided into two groups (excellent group and bad group) according to the training scores after 4 days' Y-maze learning training.The expression of cav-1 protein in cortex and hippocampus of the mice with different scores were measured through Western blot technology.Results Cav-1 protein expression of excellent group( Cav-1/β-actin was 5.71 + 1.11 ) in the cortex was significantly higher than that of the bad group ( Cav-1/β-actin was 1.69 + 0.20) (P ＜ 0.01 ).While there was no significant difference in the two groups in the expression of Cav-1 protein in hippocampus(P ＞ 0.05 ).Conclusion The operation scores have distinct correlation with the expression of Cav-1 protein in the cortex of mice.