国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2009年
1期
69-72
,共4页
杨锡贵%栾祖鹏%张化芝%湛玉静%田庆美
楊錫貴%欒祖鵬%張化芝%湛玉靜%田慶美
양석귀%란조붕%장화지%담옥정%전경미
癌,非小细胞肺%Survivin%主要组织相容性复合物
癌,非小細胞肺%Survivin%主要組織相容性複閤物
암,비소세포폐%Survivin%주요조직상용성복합물
Carcinoma,non-small-cell lung%Survivin%Major histocompatibility complex
目的 研究非小细胞肺癌(NSCLC)组织和正常组织中Survivin和主要组织相容性复合体(MnC)的表达情况,以及相互之间的关系.方法 应用流式细胞仪定量检测4JD例NSCLC组织、15例正常肺组织中Survivin蛋白及MHC的表达情况.结果 NSCLC中Survivin的阳性表达率在Ⅰ、Ⅱ、Ⅲ期患者中分别是28.57%、30.77%、80.00%,Ⅰ期与Ⅲ期之间及Ⅱ期与Ⅲ期差异有统计学意义(P=0.013,P=0.005);Ⅰ+Ⅱ期与Ⅲ期之间差异有统计学意义(P=0.01);淋巴结转移阳性组与阴性组之间差异有统计学意义(P=0.030);NSCLC中MHC-Ⅰ的失表达率在高分化组、中分化组、低分化组中分别是37.50%、42.10%、84.62%,低分化组明显低于高分化组及中分化组(P=0.026,P=0.016);NSCLC中MHC-Ⅱ的阳性表达率与NSCLC病理类型有关(P=0.005),鳞癌及非鳞非腺癌中MHC-Ⅱ表达均低于腺癌(P=0.002,P=0.04);NSCLC中Survivin表达与MHC-Ⅰ、MHC-Ⅱ表达无明显相关性.结论 Survivin与MHC在NSCLC的发生发展中有重要作用,联合检测有助于NSCLC患者预后的判断.
目的 研究非小細胞肺癌(NSCLC)組織和正常組織中Survivin和主要組織相容性複閤體(MnC)的錶達情況,以及相互之間的關繫.方法 應用流式細胞儀定量檢測4JD例NSCLC組織、15例正常肺組織中Survivin蛋白及MHC的錶達情況.結果 NSCLC中Survivin的暘性錶達率在Ⅰ、Ⅱ、Ⅲ期患者中分彆是28.57%、30.77%、80.00%,Ⅰ期與Ⅲ期之間及Ⅱ期與Ⅲ期差異有統計學意義(P=0.013,P=0.005);Ⅰ+Ⅱ期與Ⅲ期之間差異有統計學意義(P=0.01);淋巴結轉移暘性組與陰性組之間差異有統計學意義(P=0.030);NSCLC中MHC-Ⅰ的失錶達率在高分化組、中分化組、低分化組中分彆是37.50%、42.10%、84.62%,低分化組明顯低于高分化組及中分化組(P=0.026,P=0.016);NSCLC中MHC-Ⅱ的暘性錶達率與NSCLC病理類型有關(P=0.005),鱗癌及非鱗非腺癌中MHC-Ⅱ錶達均低于腺癌(P=0.002,P=0.04);NSCLC中Survivin錶達與MHC-Ⅰ、MHC-Ⅱ錶達無明顯相關性.結論 Survivin與MHC在NSCLC的髮生髮展中有重要作用,聯閤檢測有助于NSCLC患者預後的判斷.
목적 연구비소세포폐암(NSCLC)조직화정상조직중Survivin화주요조직상용성복합체(MnC)적표체정황,이급상호지간적관계.방법 응용류식세포의정량검측4JD례NSCLC조직、15례정상폐조직중Survivin단백급MHC적표체정황.결과 NSCLC중Survivin적양성표체솔재Ⅰ、Ⅱ、Ⅲ기환자중분별시28.57%、30.77%、80.00%,Ⅰ기여Ⅲ기지간급Ⅱ기여Ⅲ기차이유통계학의의(P=0.013,P=0.005);Ⅰ+Ⅱ기여Ⅲ기지간차이유통계학의의(P=0.01);림파결전이양성조여음성조지간차이유통계학의의(P=0.030);NSCLC중MHC-Ⅰ적실표체솔재고분화조、중분화조、저분화조중분별시37.50%、42.10%、84.62%,저분화조명현저우고분화조급중분화조(P=0.026,P=0.016);NSCLC중MHC-Ⅱ적양성표체솔여NSCLC병리류형유관(P=0.005),린암급비린비선암중MHC-Ⅱ표체균저우선암(P=0.002,P=0.04);NSCLC중Survivin표체여MHC-Ⅰ、MHC-Ⅱ표체무명현상관성.결론 Survivin여MHC재NSCLC적발생발전중유중요작용,연합검측유조우NSCLC환자예후적판단.
Objective To explore the expression of Survivin and MHC in non-small cell lung cancer (NSCLC), and their interaction.Methods A total of 40 patients with histologically diagnosed NSCLC were enrolled in this study.Control samples were consisted of normal lung tissues from 15 patients.Expression of Survivin and MHC were detected by flow cytometry in 40 non-small cell lung cancer tissues and 15 normal lung tissues.Results The positive rates of Survivin protein expression in NSCLC tissues classified stages Ⅰ,Ⅱ and Ⅲ were 28.57% ,30.77% and 80.00% respectively.There was a correlation between Survivin protein expres-sion and stages of NSCLC.Survivin protein expression was detected in 19 of 29 patients with lymph node metas-tasis, and 3 of 11 patients with no metastasis.There was a statistically significant difference between the two groups (P<0.030).The loss expression rates of MHC-Ⅰ in NSCLC tissues of low grade,intermediate grade and high grade were 84.62% ,42.10% and 37.50% respectively.There was a correlation in expression be-tween MHC-Ⅰ and tumor grade.The positive rates of MHC-Ⅱ expression in NSCLC tissues was related to pa-thology type (P=0.005).The expression in squamous cancer and non-squamous non-adno cancer was lower than that of adenocarcinoma (P=0.002, P=0.04).There was no obvious correlation between the expression of Survivin and MHC-Ⅰ,MHC-Ⅱ in NSCLC.Conclution The expression of Survivin and MHC could be in-volved in the pathogenesis and development of NSCLC, and the combined detection will predict the prognosis of the patients with NSCLC.
