国际免疫学杂志
國際免疫學雜誌
국제면역학잡지
INTERNATIONAL JOURNAL OF IMMUNOLOGY
2011年
6期
381-383,396
,共4页
刘洁%李伟%姜文静%贾天军%常晓彤
劉潔%李偉%薑文靜%賈天軍%常曉彤
류길%리위%강문정%가천군%상효동
MASP-2N%RT-PCR%基因克隆%原核表达
MASP-2N%RT-PCR%基因剋隆%原覈錶達
MASP-2N%RT-PCR%기인극륭%원핵표체
MASP-2N%RT-PCR%G ene cloning%Prokaryotic expression
目的 克隆并表达人MASP-2N端片段,为制备单克隆抗体及其临床应用奠定基础.方法 采用RT-PCR技术从人胎肝组织总RNA中扩增人MASP-2N端cDNA,克隆入pGEX-6p-2表达载体,在E.coli中表达GST- MASP-2N融合蛋白.结果 酶切图谱分析和DNA测序分析表明,成功构建了含人MASP-2N端片段cDNA的重组质粒,并在大肠杆菌中表达了人MASP-2N端多肽片段.结论 成功克隆表达了人MASP-2N端片段.
目的 剋隆併錶達人MASP-2N耑片段,為製備單剋隆抗體及其臨床應用奠定基礎.方法 採用RT-PCR技術從人胎肝組織總RNA中擴增人MASP-2N耑cDNA,剋隆入pGEX-6p-2錶達載體,在E.coli中錶達GST- MASP-2N融閤蛋白.結果 酶切圖譜分析和DNA測序分析錶明,成功構建瞭含人MASP-2N耑片段cDNA的重組質粒,併在大腸桿菌中錶達瞭人MASP-2N耑多肽片段.結論 成功剋隆錶達瞭人MASP-2N耑片段.
목적 극륭병표체인MASP-2N단편단,위제비단극륭항체급기림상응용전정기출.방법 채용RT-PCR기술종인태간조직총RNA중확증인MASP-2N단cDNA,극륭입pGEX-6p-2표체재체,재E.coli중표체GST- MASP-2N융합단백.결과 매절도보분석화DNA측서분석표명,성공구건료함인MASP-2N단편단cDNA적중조질립,병재대장간균중표체료인MASP-2N단다태편단.결론 성공극륭표체료인MASP-2N단편단.
Objective To clone and express the human MASP-2N-terminal fragment in E.coli.Methods Total RNA was extracted from human fetal liver tissue and MASP-2N cDNA was amplified by RT-PCR.The PCR product was cloned into pGEX-6p-2 vector.E.coli was transformed by the recombinant plasmid and GST- MASP-2N fusion protein was expressed.Results The constructed recombinant plasmids were identified by enzyme digestion and DNA sequencing.The recombinant protein was expressed in E.coli.Conclusion The N-terminal fragment of human MASP-2 was cloned and expressed.
