CAJ | 학술논문

目的探讨异丙酚对抑郁大鼠电休克治疗后海马神经元环氧化酶-2(COX-2)表达的影响.方法健康成年雄性SD大鼠50只,2～3月龄,体重200 ～ 250 g,采用连续不可预见性慢性应激制备抑郁模型,造模成功的40只大鼠,采用随机数字表法,将其随机分为4组(n=10):抑郁组(D组)、异丙酚组(P组)、电休克组(E组)和异丙酚联合电休克组(PE组),另取10只大鼠作为正常对照组(C组).P组和PE组腹腔注射异丙酚80 mg/kg,其他3组给予等容量生理盐水,E组和PE组分别于给予生理盐水8 ml/kg或异丙酚后5 min时进行电休克治疗,1次/d,连续7d.分别于造模前、造模后和治疗结束后进行进行水迷宫实验,记录逃避潜伏期和目标象限游泳时间百分比.然后处死大鼠,取海马组织,检测COX-2表达.结果与C组比较,D组、P组、E组和PE组造模后逃避潜伏期延长,目标象限游泳时间百分比降低,海马组织COX-2表达上调,D组、P组和E组治疗结束后逃避潜伏期延长,目标象限游泳时间百分比降低(P＜0.05);与D组比较,E组治疗结束后逃避潜伏期延长,海马组织COX-2表达上调,PE组逃避潜伏期缩短,目标象限游泳时间百分比升高(P＜0.05);与E组比较,PE组治疗结束后逃避潜伏期缩短,目标象限游泳时间百分比升高,海马组织COX-2表达下调(P＜0.05).结论异丙酚可改善抑郁大鼠电休克治疗后认知功能,其机制与下调海马神经元COX-2表达有关.
목적 탐토이병분대억욱대서전휴극치료후해마신경원배양화매-2(COX-2)표체적영향.방법 건강성년웅성SD대서50지,2～3월령,체중200 ～ 250 g,채용련속불가예견성만성응격제비억욱모형,조모성공적40지대서,채용수궤수자표법,장기수궤분위4조(n=10):억욱조(D조)、이병분조(P조)、전휴극조(E조)화이병분연합전휴극조(PE조),령취10지대서작위정상대조조(C조).P조화PE조복강주사이병분80 mg/kg,기타3조급여등용량생리염수,E조화PE조분별우급여생리염수8 ml/kg혹이병분후5 min시진행전휴극치료,1차/d,련속7d.분별우조모전、조모후화치료결속후진행진행수미궁실험,기록도피잠복기화목표상한유영시간백분비.연후처사대서,취해마조직,검측COX-2표체.결과 여C조비교,D조、P조、E조화PE조조모후도피잠복기연장,목표상한유영시간백분비강저,해마조직COX-2표체상조,D조、P조화E조치료결속후도피잠복기연장,목표상한유영시간백분비강저(P＜0.05);여D조비교,E조치료결속후도피잠복기연장,해마조직COX-2표체상조,PE조도피잠복기축단,목표상한유영시간백분비승고(P＜0.05);여E조비교,PE조치료결속후도피잠복기축단,목표상한유영시간백분비승고,해마조직COX-2표체하조(P＜0.05).결론 이병분가개선억욱대서전휴극치료후인지공능,기궤제여하조해마신경원COX-2표체유관.
Objective To investigate the effect of propofol on cyclooxygenase-2 (COX-2) expression in hippocampal neurons in depressed rats after electroconvulsive therapy (ECT).Methods Fifty 2-3 months old male SD rats weighing 200-250 g were randomly divided into 5 groups (n =10 each):group control (group C) ; group depression (group D); group propofol (group P); group ECT (group E) and group propofol + ECT (group PE).Depression was induced by separation and chronic unpredictable mild stres in groups D,P,E and PE.Groups P and PE received intraperitoneal pro pofol 80 mg/kg.Groups E and PE received ECT at 5 min after IP normal saline 8 ml/kg and propofol 80 mg/kg respectively once a day for 7 consecutive days.The learning and memory function was assessed by using Morris water maze test before (baseline) and after depression was induced and ECT.The animals were then sacrificed and their brains removed for detection of COX-2 mRNA expression in hippocampus (by RT-PCR).Results In group D depression significantly prolonged evasive latency and decreased swimming time percentage in platform quadrant and up-regulated COX-2 mRNA expression as compared with group C.In group E ECT further prolonged evasive latency and up-regulated COX-2 mRNA expression in depressed rats.In group PE propofol pretreatment attenuated ECT-induced impairment of learning-memory function and increase in COX-2 mRNA expression as compared with group E.Conclusion Propofol can ameliorate the decrease in learning and memory function induced by ECT in depressed rats by inhibiting COX-2 expression in hippocampal neurons.