CAJ | 학술논문

不同植物花粉的自身特性不同,导致不同的染色方法只适合某些植物花粉生活力的测定。以能够在河北省石家庄地区自然开花结实的甘薯品种河北351及其不同的自交后代材料为试材,利用目前常用的4种花粉染色法（I-KI染色法、TTC染色法、过氧化物酶染色法和蓝墨水染色法）分别测定甘薯花粉的生活力,以筛选出适宜的甘薯花粉生活力测定方法;并利用该方法,对河北351自交各世代群体中自然开花株系的花粉生活力进行检测,以验证方法的可行性以及检测结果的可靠性。结果表明：适宜甘薯花粉生活力检测方法为TTC染色法,且方法可行、结果可靠。该方法测定甘薯花粉生活力具有操作简便快捷、成本低、适合大批量检测等优点,可为开展甘薯亲和性相关研究奠定基础。
불동식물화분적자신특성불동,도치불동적염색방법지괄합모사식물화분생활력적측정。이능구재하북성석가장지구자연개화결실적감서품충하북351급기불동적자교후대재료위시재,이용목전상용적4충화분염색법（I-KI염색법、TTC염색법、과양화물매염색법화람묵수염색법）분별측정감서화분적생활력,이사선출괄의적감서화분생활력측정방법;병이용해방법,대하북351자교각세대군체중자연개화주계적화분생활력진행검측,이험증방법적가행성이급검측결과적가고성。결과표명：괄의감서화분생활력검측방법위TTC염색법,차방법가행、결과가고。해방법측정감서화분생활력구유조작간편쾌첩、성본저、괄합대비량검측등우점,가위개전감서친화성상관연구전정기출。
The sweet potato variety Hebei 351 and its self-crossed progeny were used as the materials,which was naturally flowering and fruiting in Shijiazhuang.The pollens were stained by four methods（I-KI method,TTC method,peroxide enzyme method and blue-ink method）to screen the suitable one.The feasibility and reliability were tested.The results showed that TTC method was suitable for sweet potato with reliability as well as feasibility.The method had some advantages such as simple,rapid,low cost and suitable for mass serial detection and so on,which laid a foundation for further study on the compatibility of sweet potato.