CAJ | 학술논문

[目的]筛选胡桃的EST-SSR分子标记并对其进行引物设计。[方法]利用生物信息学方法对NCBI网上公开的5213条胡桃（Juglans regia Linn.）ESTs序列进行EST-SSR特征分析,利用利用Primer3.0软件对筛选出的EST序列设计引物。[结果]在ESTs序列中共检索出207个SSR,其中无冗余序列188,检出率为3.97%,平均分布距离为21.12kb,包括92种重复基元,其中以二核苷酸和三核苷酸重复类型为主,分别占总数目的31.40%和35.27%;对随机筛选出的50条SSR引物进行PCR扩增验证,初步筛选出30对引物。[结论]通过对胡桃EST序列中SSR位点的发掘分析,有针对性地设计EST-SSR引物,将为胡桃科EST-SSR分子标记的开发奠定基础。
[목적]사선호도적EST-SSR분자표기병대기진행인물설계。[방법]이용생물신식학방법대NCBI망상공개적5213조호도（Juglans regia Linn.）ESTs서렬진행EST-SSR특정분석,이용이용Primer3.0연건대사선출적EST서렬설계인물。[결과]재ESTs서렬중공검색출207개SSR,기중무용여서렬188,검출솔위3.97%,평균분포거리위21.12kb,포괄92충중복기원,기중이이핵감산화삼핵감산중복류형위주,분별점총수목적31.40%화35.27%;대수궤사선출적50조SSR인물진행PCR확증험증,초보사선출30대인물。[결론]통과대호도EST서렬중SSR위점적발굴분석,유침대성지설계EST-SSR인물,장위호도과EST-SSR분자표기적개발전정기출。
[Objective] This research aimed to develop walnut EST-SSR markers and design corresponding primers.[Method] 5213 EST sequences of walnut（Juglans regia Linn.） public online in NCBI were used for character analysis with bioinformatics methods,and primers were designed for the selected EST sequences by using Primer 3.0 software.[Result] 207 SSRs were obtained from the EST sequences,including 188 non-redundant sequences,the detection rate was 3.97% with an average distribution distance of 21.12 kb.Totally 92 types of repeat motifs were involved,which were mainly composed of dinucleotide and trinucleotide,accounting for 31.40% and 35.27% of the total number of repeat motifs,respectively.30 pairs of primers were initially selected from the 50 randomly-selected SSR primers by PCR amplification.[Conclusion] This research would lay foundations for the development of EST-SSR molecular markers in walnut and design of the targeted EST-SSR primers by mining and analyzing the SSR sites in walnut EST sequences.