肉类工业
肉類工業
육류공업
MEAT INDUSTRY
2011年
11期
21-24
,共4页
胶原蛋白酶%牛骨%胶原蛋白%胶原多肽%酶解%动力学
膠原蛋白酶%牛骨%膠原蛋白%膠原多肽%酶解%動力學
효원단백매%우골%효원단백%효원다태%매해%동역학
collagenase%bovine bone%collagen protein%collagen polypetides%enzymolysis%kinetics
针对从细菌中提取的胶原蛋白酶酶解牛骨胶原蛋白的动力学性质进行研究。通过全波长扫描和SDS—PAGE电泳，对酶解的动态变化及其多肽分子量的变化进行了研究，考察了温度、酶用量、底物浓度等因素对酶解动态的影响，并对酶解的过程动力学进行了分析。结果说明：胶原蛋白酶酶解牛骨胶原蛋白得到的多肽分子量分布不均匀，分子量大小随反应时间的增大而减小；在一定范围内，增大酶浓度、提高温度可使酶解反应更彻底。
針對從細菌中提取的膠原蛋白酶酶解牛骨膠原蛋白的動力學性質進行研究。通過全波長掃描和SDS—PAGE電泳，對酶解的動態變化及其多肽分子量的變化進行瞭研究，攷察瞭溫度、酶用量、底物濃度等因素對酶解動態的影響，併對酶解的過程動力學進行瞭分析。結果說明：膠原蛋白酶酶解牛骨膠原蛋白得到的多肽分子量分佈不均勻，分子量大小隨反應時間的增大而減小；在一定範圍內，增大酶濃度、提高溫度可使酶解反應更徹底。
침대종세균중제취적효원단백매매해우골효원단백적동역학성질진행연구。통과전파장소묘화SDS—PAGE전영，대매해적동태변화급기다태분자량적변화진행료연구，고찰료온도、매용량、저물농도등인소대매해동태적영향，병대매해적과정동역학진행료분석。결과설명：효원단백매매해우골효원단백득도적다태분자량분포불균균，분자량대소수반응시간적증대이감소；재일정범위내，증대매농도、제고온도가사매해반응경철저。
Enzymolysis dynamics of bovine bone collagen hydrolyzeO Dy conagenase cx,ta bacteria was studied. Through the full wavelength scan and SDS -PAGE, the dynamic changes of the enz- ymolysis and molecular weight of collagen polypeptide were researched. Effects of temperature, enzyme amount, substrate concentrationed on enzymolysis dynamics, and changes of enzyme kinetics were analyzed. The results showed that molecular weight of the collagen polypeptide obtained from bovine bone collagen digested by collagenase was non - uniform distribution; molecular weight was decreased with reaction time increased; at a certain range, enzymolysis reacted more completely along with enzyme concentration and temperature increasing.
