延边大学学报：自然科学版
延邊大學學報：自然科學版
연변대학학보：자연과학판
Journal of Yanbian University：Natural Science
2011年
2期
152-155
,共4页
固相萃取%高效液相色谱法%五仁醇%木脂素
固相萃取%高效液相色譜法%五仁醇%木脂素
고상췌취%고효액상색보법%오인순%목지소
SPE%HPLC%nuts alcohol%lignans
建立了五仁醇浸膏中5种木质素（五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素）的快速检测方法,并采用固相萃取高效液相色谱法对其进行了检测.结果显示：五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素进样量分别在0.048 8-2.44、0.040 8-2.04、0.049 6-2.48、0.036 0-1.80和0.048 0-2.40μg范围内具有良好的线性关系（R^2≤0.999 8）;平均加样回收率分别为101.24%、99.52%、103.72%、98.66%和98.08%,RSD≤1.62%.实验表明,固相萃取-高效液相色谱法具有简便、准确、稳定、灵敏度高、重现性好等优点,可用于五仁醇浸膏的质量控制.
建立瞭五仁醇浸膏中5種木質素（五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素）的快速檢測方法,併採用固相萃取高效液相色譜法對其進行瞭檢測.結果顯示：五味子醇甲、五味子醇乙、五味子脂甲、五味子甲素和五味子乙素進樣量分彆在0.048 8-2.44、0.040 8-2.04、0.049 6-2.48、0.036 0-1.80和0.048 0-2.40μg範圍內具有良好的線性關繫（R^2≤0.999 8）;平均加樣迴收率分彆為101.24%、99.52%、103.72%、98.66%和98.08%,RSD≤1.62%.實驗錶明,固相萃取-高效液相色譜法具有簡便、準確、穩定、靈敏度高、重現性好等優點,可用于五仁醇浸膏的質量控製.
건립료오인순침고중5충목질소（오미자순갑、오미자순을、오미자지갑、오미자갑소화오미자을소）적쾌속검측방법,병채용고상췌취고효액상색보법대기진행료검측.결과현시：오미자순갑、오미자순을、오미자지갑、오미자갑소화오미자을소진양량분별재0.048 8-2.44、0.040 8-2.04、0.049 6-2.48、0.036 0-1.80화0.048 0-2.40μg범위내구유량호적선성관계（R^2≤0.999 8）;평균가양회수솔분별위101.24%、99.52%、103.72%、98.66%화98.08%,RSD≤1.62%.실험표명,고상췌취-고효액상색보법구유간편、준학、은정、령민도고、중현성호등우점,가용우오인순침고적질량공제.
A rapid and simple method based on solid phase extraction（SPE） combined with HPLC was developed and validated for simultaneous determination of five lignans（Schizandrin,Schizandrol B,Schizantherin A,Deoxyschizandrin and Schizandrin B） from Nuts Alcohol.The Schizandrin,Schizandrol B,Schizantherin A,Deoxyschizandrin and Schizandrin B were showed good linearity（R^2≤0.9998） within range of 0.0488-2.44,0.0408-2.04,0.0496-2.48,0.0360-1.80 and 0.0480-2.40μg,and the average recoveries were 101.24%,99.52%,103.72%,98.66% and 98.08% with low relative standard deviation（RSD≤1.62 %）,respectively.The results shown that the SPE-HPLC is a convenient,sensitive,robustness,reproducible and accurate protocol,it can be routinely used in the quality control of Nuts Alcohol.
