CAJ | 학술논문

本研究采用逐量分批驯化的方法,从造纸废水中分离得到一株能够以苯酚为唯一碳源生长的苯酚降解菌株F5-1。经形态观察、生理生化特性鉴定及16S rDNA序列分析,将该菌株鉴定为克雷伯菌（Klebsiella sp.）。该菌株能够在7h时完全降解初始浓度为100mg/L的苯酚,降解苯酚主要发生在生长对数期;在pH5.0～9.0,NaCl浓度0～80g/L,温度20～40℃范围内,菌株F5-1均可有效降解初始浓度为100～1200mg/L的苯酚;能够耐受的最大苯酚浓度为1500mg/L。本研究结果表明,F5-1菌株对处理环境条件复杂的含酚废水具有潜在的应用前景。
본연구채용축량분비순화적방법,종조지폐수중분리득도일주능구이분분위유일탄원생장적분분강해균주F5-1。경형태관찰、생리생화특성감정급16S rDNA서렬분석,장해균주감정위극뢰백균（Klebsiella sp.）。해균주능구재7h시완전강해초시농도위100mg/L적분분,강해분분주요발생재생장대수기;재pH5.0～9.0,NaCl농도0～80g/L,온도20～40℃범위내,균주F5-1균가유효강해초시농도위100～1200mg/L적분분;능구내수적최대분분농도위1500mg/L。본연구결과표명,F5-1균주대처리배경조건복잡적함분폐수구유잠재적응용전경。
By gradually increasing the concentration of phenol in the culture medium,a phenol-degrading bacterial strain designated as F5-1 was isolated from the wastewater of a paper mill.The organism was able to utilize phenol as a sole carbon source.Based on morphological,physiological and biochemical characterizations and 16S rDNA sequence analysis,F5-1 was identified as a species of Klebsiella.The strain F5-1 could degrade phenol with initial concentration of 100 mg/L completely in 7 h and the phenol degradation was found to occur in log phase.At pH 5.0～9.0,the strain F5-1 could efficiently degrade phenol with initial concentration from 100 mg/L to 1 200 mg/L in the presence of 0～80 g/L of NaCl at temperature range from 20℃ to 40℃.The highest concentration of phenol that the strain can tolerate was 1 500 mg/L.These results indicated that the strain F5-1 may have potential application in treatment of phenol-containing wastewater with complex environmental conditions.