现代食品科技
現代食品科技
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MODERN FOOD SCIENCE AND TECHNOLOGY
2010年
11期
1283-1288
,共6页
刘晓云%何小维%王国洪%Christine Moore%Cynthia Counter%杨连生%Michael Vincent%James Soares
劉曉雲%何小維%王國洪%Christine Moore%Cynthia Counter%楊連生%Michael Vincent%James Soares
류효운%하소유%왕국홍%Christine Moore%Cynthia Counter%양련생%Michael Vincent%James Soares
莱克多巴胺%SPE%衍生化%GUMS%LC/MS/1V4S%灵敏度
萊剋多巴胺%SPE%衍生化%GUMS%LC/MS/1V4S%靈敏度
래극다파알%SPE%연생화%GUMS%LC/MS/1V4S%령민도
ractopamine%SPE%derivatization%GUMS%sensitivity
本实验探讨影响GUMS以及LC/MS/MS检测灵敏度的影响因素.对GUMS法的固相萃取(SPE)和衍生化条件进行优化,测试了SPE、加热时间、温度、溶液凡式归匕等因素.同时测试这些条件对LUMC/MC法的灵敏度.结果表明,碱基化为影响GUMS法灵敏度的主要因素,能提高灵敏度,对衍生化条件也有影响.通过对样本进行固相萃取,速日处理,GUMS法的灵敏度为0.5 ng/mL,LC/MC/MC法的为0.25 ng/mL.采用这种更灵敏的方法,尿液中莱克多巴胺的测试窗口期可由2d提高到6d.经过优化的GUMS法及LC/MS/MS方法灵敏度更高,适合猪尿中莱克多巴胺的检测.
本實驗探討影響GUMS以及LC/MS/MS檢測靈敏度的影響因素.對GUMS法的固相萃取(SPE)和衍生化條件進行優化,測試瞭SPE、加熱時間、溫度、溶液凡式歸匕等因素.同時測試這些條件對LUMC/MC法的靈敏度.結果錶明,堿基化為影響GUMS法靈敏度的主要因素,能提高靈敏度,對衍生化條件也有影響.通過對樣本進行固相萃取,速日處理,GUMS法的靈敏度為0.5 ng/mL,LC/MC/MC法的為0.25 ng/mL.採用這種更靈敏的方法,尿液中萊剋多巴胺的測試窗口期可由2d提高到6d.經過優化的GUMS法及LC/MS/MS方法靈敏度更高,適閤豬尿中萊剋多巴胺的檢測.
본실험탐토영향GUMS이급LC/MS/MS검측령민도적영향인소.대GUMS법적고상췌취(SPE)화연생화조건진행우화,측시료SPE、가열시간、온도、용액범식귀비등인소.동시측시저사조건대LUMC/MC법적령민도.결과표명,감기화위영향GUMS법령민도적주요인소,능제고령민도,대연생화조건야유영향.통과대양본진행고상췌취,속일처리,GUMS법적령민도위0.5 ng/mL,LC/MC/MC법적위0.25 ng/mL.채용저충경령민적방법,뇨액중래극다파알적측시창구기가유2d제고도6d.경과우화적GUMS법급LC/MS/MS방법령민도경고,괄합저뇨중래극다파알적검측.
To get a high-sensitivity method for detection of ractopamine in urine samples of pigs,SPE and denvatiztion conditions wereoptimized.Among the examined factors including SPE,heating time,temperature,solution and neutralization,neutralization was found as themost important to GUMS sensitivity,which affected the dervatization conditions and improved the sensitivity.The sensitivities of GUMS andLC/MC/MC were 0.5 ng/mL and 0.25 ng/mL,respectively.By this higher sensitivity method,the detection window of ractopamine in urine canbe improved from 2 days up to 6 days.
