中国医药生物技术
中國醫藥生物技術
중국의약생물기술
CHINESE MEDICINAL BIOTECHNOLOGY
2010年
1期
24-27,封1
,共5页
李丹%毕龙%刘民%金静%吕荣%王军%白建萍%吴拮%李静%刘崟%胡蕴玉
李丹%畢龍%劉民%金靜%呂榮%王軍%白建萍%吳拮%李靜%劉崟%鬍蘊玉
리단%필룡%류민%금정%려영%왕군%백건평%오길%리정%류음%호온옥
骨形态发生蛋白质类%成骨细胞%破骨细胞%异位成骨%骨诱导
骨形態髮生蛋白質類%成骨細胞%破骨細胞%異位成骨%骨誘導
골형태발생단백질류%성골세포%파골세포%이위성골%골유도
Bone morphogenetic proteins%Osteoblasts%Osteoclasts%Ectopic bone formation%Osteoinduction
目的 长期观察并获得单纯牛骨形态发生蛋白(bovine bonemorphogenetic protein,bBMP)异位诱导成骨的组织学变化.方法 (20±2)g昆明小鼠21只,麻醉后于双侧股部肌肉中直接植入块状bBMP各2mg,分别于第1、2、4、6、8、10、12周各处死3只,切取诱导分化组织,5%戊二醛固定,标本用10%EDTA脱钙后制作5μm石蜡切片,分别行甲苯胺蓝染色,HE染色,丽春红三色染色观察组织学变化情况.结果 植入1周,大量分化良好的间充质细胞和幼稚软骨细胞形成一椭圆形分化组织块;植入2周,大量骨小梁生成,部分骨小梁边缘骨化;植入4周,骨小梁转化为成熟的骨质,骨质中有散在于骨细胞中的破骨细胞生成;植入6～12周,椭圆形骨组织块内部小梁骨逐渐吸收、断裂、不连接,但外周骨质逐渐塑形,局部成熟骨质周边仍伴有新生骨形成.结论 bBMP无需任何载体即可在肌肉中异位成骨,具有强大的异位骨诱导能力;血供在异位骨的形成和维持中可能起着重要作用.
目的 長期觀察併穫得單純牛骨形態髮生蛋白(bovine bonemorphogenetic protein,bBMP)異位誘導成骨的組織學變化.方法 (20±2)g昆明小鼠21隻,痳醉後于雙側股部肌肉中直接植入塊狀bBMP各2mg,分彆于第1、2、4、6、8、10、12週各處死3隻,切取誘導分化組織,5%戊二醛固定,標本用10%EDTA脫鈣後製作5μm石蠟切片,分彆行甲苯胺藍染色,HE染色,麗春紅三色染色觀察組織學變化情況.結果 植入1週,大量分化良好的間充質細胞和幼稚軟骨細胞形成一橢圓形分化組織塊;植入2週,大量骨小樑生成,部分骨小樑邊緣骨化;植入4週,骨小樑轉化為成熟的骨質,骨質中有散在于骨細胞中的破骨細胞生成;植入6～12週,橢圓形骨組織塊內部小樑骨逐漸吸收、斷裂、不連接,但外週骨質逐漸塑形,跼部成熟骨質週邊仍伴有新生骨形成.結論 bBMP無需任何載體即可在肌肉中異位成骨,具有彊大的異位骨誘導能力;血供在異位骨的形成和維持中可能起著重要作用.
목적 장기관찰병획득단순우골형태발생단백(bovine bonemorphogenetic protein,bBMP)이위유도성골적조직학변화.방법 (20±2)g곤명소서21지,마취후우쌍측고부기육중직접식입괴상bBMP각2mg,분별우제1、2、4、6、8、10、12주각처사3지,절취유도분화조직,5%무이철고정,표본용10%EDTA탈개후제작5μm석사절편,분별행갑분알람염색,HE염색,려춘홍삼색염색관찰조직학변화정황.결과 식입1주,대량분화량호적간충질세포화유치연골세포형성일타원형분화조직괴;식입2주,대량골소량생성,부분골소량변연골화;식입4주,골소량전화위성숙적골질,골질중유산재우골세포중적파골세포생성;식입6～12주,타원형골조직괴내부소량골축점흡수、단렬、불련접,단외주골질축점소형,국부성숙골질주변잉반유신생골형성.결론 bBMP무수임하재체즉가재기육중이위성골,구유강대적이위골유도능력;혈공재이위골적형성화유지중가능기착중요작용.
Objective To observe and get the histological changes of the ectopic bone induced by the bBMP during a long period. Methods Twenty one Kunming rats (20 ± 2) g were used in this experiment, 2 mg bBMP was implanted into the rat intramuscular of both sides of the thigh under the anesthesia respectively, differentiated tissues were harvested at 1, 2, 4, 6, 8, 10 and 12 weeks postoperation. The incised tissues were immersion-fixed in 5% glutaraldehyde, samples were subsequently decalcified in 10% EDTA and processed for paraffin sectioning at 5 μm, sections were stained with toluidiue blue, hematoxylin and eosin, Masson trichrome. Results One week after the operation, an oval differentiated tissue consisting of a large number of chundroblaats and chondrocytes was formed; two weeks after the operation, a large number of bone trabecular was formed newly and the verge of the trabecular had been mineralized; four weeks after the operation, most of the newly formed bone became more mature, and simultaneously a few osteoclasts were found among the osteoblasts at this period; from 6 weeks to 12 weeks, the inner wabecular in the oval ectopic bone lump was partly absorbed, as a result, the trabecular became rupture and disconnected, but the outer ectopic bone was not found to be absorbed, some newly bone still formed around the mature bone. Conclusions bBMP possesse a strong ectopic osteoinductivity, having the potential for ectopic new bone formation without any carder. The blood supply may have an important role in producing the new bone and maintaining its existence.
