CAJ | 학술논문

对采自不同产地\,不同年份黑骨藤的总黄酮含量进行了测定.以芦丁为对照品,检测波长510 nm,绘制标准曲线,采用紫外分光光度法测定总黄酮的相对百分含量.芦丁对照品在2.065～103.250 mg·L~(-1)范围内有良好的线性关系(r=0.999 90),平均回收率为98.03%,RSD值为1.40%.采用分光光度法测定黑骨藤的总黄酮含量操作简便\,结果可靠,为评价黑骨藤的质量提供了依据.
대채자불동산지\,불동년빈흑골등적총황동함량진행료측정.이호정위대조품,검측파장510 nm,회제표준곡선,채용자외분광광도법측정총황동적상대백분함량.호정대조품재2.065～103.250 mg·L~(-1)범위내유량호적선성관계(r=0.999 90),평균회수솔위98.03%,RSD치위1.40%.채용분광광도법측정흑골등적총황동함량조작간편\,결과가고,위평개흑골등적질량제공료의거.
Determination of the total flavonoids in Periploca forrestii Schltr of different areas and different years has been performed in this paper. The percentage of total flavonoids was determined by ultraviolet spectrophotometry at 510 nm based on the standard curve, with rutin as controlled sample. There was good linearity (r=0.999 27) during the range of 2.065～103.250 mg·L~(-1)for rutin. The average recovery was 98.03% and the RSD was 1.40%. The content of total flavonoids in Periploca forrestii Schltr of different areas and different years was measured. The ultraviolet spectrophotometry method is simple and reliable to measure the content of total flavonoids in Periploca forrestii Schltr. The results provide the evidence for evaluating the quality of Periploca forrestii Schltr.