CAJ | 학술논문

利用铁氰化钴/树状高分子(CoHCF/PAMAM)复合材料修饰玻碳电极(GCE),制备了免标记检测基因突变的新型DNA电化学传感器.传感器中树状高分子层明显增加了单链DNA探针的同定量,铁氰化钴层增大了鸟嘌呤的氧化信号,该传感器可以灵敏识别单碱基错配的基因序列,具有良好的选择性和灵敏度.在7.6×10~(-11)～3.05×10~(-8)mol/L浓度范围内,鸟嘌呤(G)的氧化峰电流差值与突变基因浓度呈良好的线性关系,检出限为1.0×10~(-11) mol/L(S/N=3).
이용철청화고/수상고분자(CoHCF/PAMAM)복합재료수식파탄전겁(GCE),제비료면표기검측기인돌변적신형DNA전화학전감기.전감기중수상고분자층명현증가료단련DNA탐침적동정량,철청화고층증대료조표령적양화신호,해전감기가이령민식별단감기착배적기인서렬,구유량호적선택성화령민도.재7.6×10~(-11)～3.05×10~(-8)mol/L농도범위내,조표령(G)적양화봉전류차치여돌변기인농도정량호적선성관계,검출한위1.0×10~(-11) mol/L(S/N=3).
Electrochemical hybridization biosensor for label-free' determination of sequence-specific DNA was described. First, cobalt hexacyanoferrate films and poly (amidoamine) (PAMAM) dendrimer( generation 4.0) were modified on the glassy carbon electrode(GCE) sequentially. Then, DNA probes were successfully immobilized on the modified electrode with G4 PAMAM dendrimer acting as the coupling agent. The hybrid-ization events were monitored by differential pulse voltammetry(DPV) measurement based on the oxidation signals of guanine without any external labels. This biosensor exhibited a superior electrochemical stability, selectivity and sensitivity in detection of the oligonucleotide sequence. Under the optimal conditions, the guanine oxidation signal was directly proportional to the mutant gene sequence concentration from 7. 6 × 10~(-11) to 3.05 ×10~(-8) mol/L with a correlation coefficient of 0. 9975 and a detection limit of 1.0 x 10~(-11) mol/L(S/N =3).