CAJ | 학술논문

以铁兰无菌苗为外植体,研究不同激素配比对铁兰胚性愈伤组织诱导及体细胞胚形成的影响,并对体细胞胚的发生过程进行形态学观察.结果表明:2,4-D对铁兰胚性愈伤组织的诱导具有重要作用,诱导铁兰胚性愈伤组织的最适2,4-D浓度为2.0 mg/L;胚性愈伤组织在含ABA的培养基上可分化形成体细胞胚.形态及解剖学观察表明,愈伤组织既可以产生于外植体的外层细胞,又可产生于外植体的深层细胞.体细胞胚的形态发生经历了与合子胚形态发生相似的阶段,即经过球形胚、子叶形胚等.胚经过萌发、芽的伸长和生根后形成再生植株.
이철란무균묘위외식체,연구불동격소배비대철란배성유상조직유도급체세포배형성적영향,병대체세포배적발생과정진행형태학관찰.결과표명:2,4-D대철란배성유상조직적유도구유중요작용,유도철란배성유상조직적최괄2,4-D농도위2.0 mg/L;배성유상조직재함ABA적배양기상가분화형성체세포배.형태급해부학관찰표명,유상조직기가이산생우외식체적외층세포,우가산생우외식체적심층세포.체세포배적형태발생경력료여합자배형태발생상사적계단,즉경과구형배、자협형배등.배경과맹발、아적신장화생근후형성재생식주.
Shoots of Tillandsia cyanea(A. Dietrich) E. Morren were used as explants and induced and cultured on mediums supplemented with exogenous hormones to observe their somatic embryogenesis and morphological characteristics. The results showed that 2,4-D had significant effects on the formation of somatic embryogenic calli with an optimum concentration of 2,4-D of 2.0 mg/L. The embryogenic calli were developed into somatic embryos on MS added with ABA. Anatomical and morphological observations revealed that the somatic embryos were produced from both the epidermal cells and the inner cells of the explants. The somatic embryogenesis was similar to the zygotic embryogenesis, including globular embryo stage and cotyledonary embryo stage. The embryos were developed into plants through germination, shoot elongation and rooting.