CAJ | 학술논문

研究生活废水培养螺旋藻(Spirulina platensis,SP)中的主要营养物质构成.用厨房废水(kitchenwastewater,KW)摇瓶培养SP,观察藻细胞在KW中的生长状态,以及低、高光照强度(36 μmol/m~2·S和108μmol/m~2·s)对SP生长的影响.化学比色法检测KW培养的sP(KW-SP)中的总蛋白质(Pr)、脂(Lip)和水溶性多糖(Ploys)等主要营养物质的构成.结果发现,SP在KW中的生长速率、生物量及产率可达在Zarrouk培养液(ZM)中的80%以上,并表现有明显氮营养缺乏特点;与ZM培养的SP(ZM-sp)相比,低、高光照强度下培养的KW-SP中Pr(分别占干质量的47.7%和40.3%)明显降低(P<0.05),Lip(26.5%和19.3%)显著增高(P<0.05),高光照强度下培养的KW-SP中Ploys(24.1%)显著增高(P<0.05);KW-SP中天冬氨酸(Asp)和蛋氨酸含量明显增加(P<0.05),精氨酸、组氨酸和胱氨酸含量则明显降低(P<0.05).用生活废水培养的SP营养价值仍然较高,培养过程中氮缺乏和光照强度对KW-SP中蛋白质、脂、多糖及氨基酸构成有重要影响.
연구생활폐수배양라선조(Spirulina platensis,SP)중적주요영양물질구성.용주방폐수(kitchenwastewater,KW)요병배양SP,관찰조세포재KW중적생장상태,이급저、고광조강도(36 μmol/m~2·S화108μmol/m~2·s)대SP생장적영향.화학비색법검측KW배양적sP(KW-SP)중적총단백질(Pr)、지(Lip)화수용성다당(Ploys)등주요영양물질적구성.결과발현,SP재KW중적생장속솔、생물량급산솔가체재Zarrouk배양액(ZM)중적80%이상,병표현유명현담영양결핍특점;여ZM배양적SP(ZM-sp)상비,저、고광조강도하배양적KW-SP중Pr(분별점간질량적47.7%화40.3%)명현강저(P<0.05),Lip(26.5%화19.3%)현저증고(P<0.05),고광조강도하배양적KW-SP중Ploys(24.1%)현저증고(P<0.05);KW-SP중천동안산(Asp)화단안산함량명현증가(P<0.05),정안산、조안산화광안산함량칙명현강저(P<0.05).용생활폐수배양적SP영양개치잉연교고,배양과정중담결핍화광조강도대KW-SP중단백질、지、다당급안기산구성유중요영향.
Evaluation of nutrient composition of Spirulina platensis (SP) grown on domestic wastewater (DW) was undertaken. SP cultivation was carried out in digested kitchen waster (KW) either at a light flx of 36(lower )or 108 mlo photon /m~2·s(higher),utiling shaking flasks,Conterts of total protein, lipids and water soluble polysaccharides were determined by colorimetric methods. Al-though SP cultivation in DW displayed nitrogen deficiency, the special growth rate and biomass concen-tration (as dry weight) after 10 days of cultivation in the DW was above 80% to the one observed in Zar-rouk medium (ZM), regardless of the light intensity. Compared to the SP grown on ZM (ZM-SP), the protein and the total lipids contents of the biomass in SP grown on KW (KW-SP) were significantly de-creased (P<0.05), regardless of the light flux. However, the water soluble polysaccharide was obvious-ly enriched in KW-SP (21.4%) at the higher light intensity (P<0.05). Additionally, the contents of amino acids of Asp and Met were increased, while that of Arg, His and Cys were decreased in KW-SP (P<0.05). Presnt results indicated that SP products with high nutrient value could be obtained by cul-tivation in DW. The variation of nutrient compositions in KW-SP was related to the nitrogen deficiency and light intensity during the cultivation.