CAJ | 학술논문

研究了嗜酸乳杆菌的高密度培养条件,并使用喷雾干燥法对嗜酸乳杆菌粉末发酵剂进行了制备.实验表明,嗜酸乳杆菌6012的最佳增菌培养基为：乳清培养基+0.6%(w/v)低聚异麦芽糖+0.6%(w/v)黄豆粉+10%(v/v)胡萝卜汁+0.5%(w/v)CaC3.在培养过程中使用20%柠檬酸钠调节培养基pH值为6.2,37℃静置培养18 h,活菌数可达1.0×109 cfu/mL.以0.5%甘油+0.5%葡萄糖+3%大豆分离蛋白+2%海藻酸钠作为抗热保护剂,菌体经过喷雾干燥,其发酵剂活菌数为8.0×108 cfu/g.
연구료기산유간균적고밀도배양조건,병사용분무간조법대기산유간균분말발효제진행료제비.실험표명,기산유간균6012적최가증균배양기위：유청배양기+0.6%(w/v)저취이맥아당+0.6%(w/v)황두분+10%(v/v)호라복즙+0.5%(w/v)CaC3.재배양과정중사용20%저몽산납조절배양기pH치위6.2,37℃정치배양18 h,활균수가체1.0×109 cfu/mL.이0.5%감유+0.5%포도당+3%대두분리단백+2%해조산납작위항열보호제,균체경과분무간조,기발효제활균수위8.0×108 cfu/g.
The conditions of high density culture of Lactobacillus acidophilus and the production of starter powder were studied in this paper. The optimal culture conditions of L. acidophilus 6012 were obtained as followed: whey medium, 0. 6% (w/v) isomaltooligosaccharide, 0. 6% (w/v) soybean, 10%(v/v) carrot juice and 0. 5%(w/v) CaCO3. pH was adjusted to 6. 2 by addition of 20% citric acid sodium. The viable number could reach 8. 9 × 108 cfu/mL after cultured for 18 h at 37℃. The optimal protective agents were 0. 5% glycerol, 0. 5% glucose, 3% isolate soy protein and 2% sodium alginate. Under these conditions, the viable number of L. acidophilus in starter powder was 8. 0 × 108 cfu/g after spray drying.