CAJ | 학술논문

目的:同时测定獐牙菜属植物中四种抗肝炎活性成分的含量.方法:用高效液相色谱法对獐牙菜苦苷、芒果苷、当药醇苷和雏菊叶龙胆酮四种抗肝炎活性成分进行了分析.结果:在选定的色谱条件下四种成分分离良好,在检测浓度范围内线性良好.日内和日间精密度分别小于2%和4.60,加样回收率为97.94%～99.84%.建立了獐牙菜属植物中四种不同化学结构类型抗肝炎活性成分的含量测定方法.结论:本方法简单.准确,能成功的的运用于同时测定獐牙菜属植物不同部位中獐牙菜苦苷、芒果苷、当药醇苷和雏菊叶龙胆酮四种抗肝炎活性成分的含量以及獐牙菜属植物的鉴定.
목적:동시측정장아채속식물중사충항간염활성성분적함량.방법:용고효액상색보법대장아채고감、망과감、당약순감화추국협룡담동사충항간염활성성분진행료분석.결과:재선정적색보조건하사충성분분리량호,재검측농도범위내선성량호.일내화일간정밀도분별소우2%화4.60,가양회수솔위97.94%～99.84%.건립료장아채속식물중사충불동화학결구류형항간염활성성분적함량측정방법.결론:본방법간단.준학,능성공적적운용우동시측정장아채속식물불동부위중장아채고감、망과감、당약순감화추국협룡담동사충항간염활성성분적함량이급장아채속식물적감정.
AIM: To simultaneously determine four anti-hepatitis components in Swertia.METHOD: A new HPLC method using ultraviolet absorbance detection with detected wavelength at 254 nm was presented for the simul taneous determination of four active components, namely swertiamarin, mangifedn, swertianolin and bellidifolin in 7 species of Swertia plants (Gentianaceae).The samples were separated and detected on the column of Alltima C18(250 m×4.6 mm, 5 μm) under the condition of gradient elution.RESULTS:The limit of detection (S/N = 3) was less than 0.86 μg·mL-1 and the limit of quantification (S/N = 10 )was less than 1.73 μg·mL-1.All calibration curves howed good linear regression withintest ranges.The RSD for intra-and inter-day of four investigated compounds were lower than 2% and 4.6%, respectively.The mean recovery was between 97.94% and 99.84%.The method was estab lishded for the simultaneous determination of the four anti-hepatitis constituents relating to different kinds of chemical structures in Swertiaplants.CONCLUSION: The method was successfully applied to quantify the four active compoents in different parts of the herb and to identify the specific species of genus Swertia.