CAJ | 학술논문

目的观察高糖环境中大鼠肾脏系膜细胞(MC)葡萄糖转运蛋白1(G1uT-1)及转化生长因子1(TGF-β1)mRNA表达变化以及氟伐他汀(Flu)的干预作用,探讨其保护肾脏的可能机制. 方法原代培养MC分为正常对照(NG)组、甘露醇(MG)组、高糖(HG)组及HG+Flu组,RT-PCR法检测细胞中GluT-1mRNA和TGF-β1 mRNA的表达,放免法测定各组细胞培养上清液中Ⅳ型胶原含量. 结果HG组各时相点GluT-1mRNA、TGF-β1 mRNA表达量均高于NC组(P<0.01),从48h开始HG组细胞上清液Ⅳ型胶原的含量较NC组增高(P<0.05),而HG+Flu组GluT-1 mRNA表达量、TGF-β1 mRNA的表达、细胞上清液Ⅳ型胶原含量均较同时点HG组明显降低(P<0.01). 结论高糖刺激使MC内GluT-1 mRNA表达增高,TGF-β1 mRNA表达上调,Ⅳ型胶原分泌增多.高糖状态下,Flu抑制细胞外基质积聚的作用可能与其抑制高糖引起MC GIuT-1过表达、减少TGF-β1的合成有关.
목적 관찰고당배경중대서신장계막세포(MC)포도당전운단백1(G1uT-1)급전화생장인자1(TGF-β1)mRNA표체변화이급불벌타정(Flu)적간예작용,탐토기보호신장적가능궤제. 방법원대배양MC분위정상대조(NG)조、감로순(MG)조、고당(HG)조급HG+Flu조,RT-PCR법검측세포중GluT-1mRNA화TGF-β1 mRNA적표체,방면법측정각조세포배양상청액중Ⅳ형효원함량. 결과HG조각시상점GluT-1mRNA、TGF-β1 mRNA표체량균고우NC조(P<0.01),종48h개시HG조세포상청액Ⅳ형효원적함량교NC조증고(P<0.05),이HG+Flu조GluT-1 mRNA표체량、TGF-β1 mRNA적표체、세포상청액Ⅳ형효원함량균교동시점HG조명현강저(P<0.01). 결론고당자격사MC내GluT-1 mRNA표체증고,TGF-β1 mRNA표체상조,Ⅳ형효원분비증다.고당상태하,Flu억제세포외기질적취적작용가능여기억제고당인기MC GIuT-1과표체、감소TGF-β1적합성유관.
Objective To investigate the effect of fluvastatin on the expressions of GluT-1 and TGF-β1 mRNA in glomerular mesangial cells(GMC) exposed to high glucose,discuss the role of GluT1 in the onset and progression of diabetic nepropathy,and explore the possible mechanism for fluvastatin to protect renal function. Methods GMC was cultured at high glucose concentration with (HG+flu group) or without (HG group) fluvastatin. RT-PCR was used to detect GluT1 and TGF-β1 mRNA expressions, and radioimmunoassay for the content of type Ⅳcollagen in the supernatant. Results The expressions of GluT1 and TGF-β1 mRNA and the content of type Ⅳcollagen in the supernatant in GMC culture was higher in HG group than in normal glucose group. The above parameters were significantly lower in HG+flu group than in HG group. Conclusions Under high glucose stimulation,the up-regulated expression of Glu T-1 mRNA is able to induce the increased expression of TGF-β1 mRNA and the increased synthesis and secretion of type Ⅳ collagen in glomerular mesangial cells.