CAJ | 학술논문

目的研究结缔组织生长因子(connective tissue growth factor,CTGF)在日本血吸虫病肝纤维化小鼠肝脏中的表达状况及意义.方法用昆明小鼠感染尾蚴复制小鼠日本血吸虫病肝纤维化模型;RT-PCR和免疫组化分别检测小鼠肝脏CTGFmRNA和蛋白的表达,免疫组化方法观察各阶段肝脏α-SMA阳性细胞的表达.结果模型组10周时形成典型的肝纤维化,此时模型组小鼠肝脏CTGFmRNA和蛋白表达、α-SMA+细胞数达高峰,10周、14用、18用时与治疗组相比均有明显的统计学差异(P均＞0.05);CTGFmRNA和蛋白表达水平与α-SMA+细胞数均呈直线相关性.结论 CTGF基因和蛋白表达增高与小鼠日本血吸虫病肝纤维化形成有密切关系;CTGF在小鼠日本血吸虫病肝纤维化形成过程中的作用可能主要通过作用于活化的肝星状细胞来实现的.
목적 연구결체조직생장인자(connective tissue growth factor,CTGF)재일본혈흡충병간섬유화소서간장중적표체상황급의의.방법 용곤명소서감염미유복제소서일본혈흡충병간섬유화모형;RT-PCR화면역조화분별검측소서간장CTGFmRNA화단백적표체,면역조화방법 관찰각계단간장α-SMA양성세포적표체.결과 모형조10주시형성전형적간섬유화,차시모형조소서간장CTGFmRNA화단백표체、α-SMA+세포수체고봉,10주、14용、18용시여치료조상비균유명현적통계학차이(P균＞0.05);CTGFmRNA화단백표체수평여α-SMA+세포수균정직선상관성.결론 CTGF기인화단백표체증고여소서일본혈흡충병간섬유화형성유밀절관계;CTGF재소서일본혈흡충병간섬유화형성과정중적작용가능주요통과작용우활화적간성상세포래실현적.
[Objective] To study the expression and significance of connective tissue growth factor (CTGF) in murine schistosomiasis liver fibrosis. [Method] The mice infected with schistosoma japonicum were randomly divided into the model groups and the therapeutic groups. The hepatic expression of CTGF mRNA and protein was detected by reverse transcriptase polymerase chain reaction and immunohistochemical method respectively. Histological changes were observed by HE staining. The α-SMA positive cells were observed by immunohistochemical method.[Result] The expression of CTGF mRNA and protein and α-SMA positive cells in the model groups peaked at 10th week and then declined; there were significant differences at 10th, 14th, 16th week between the model groups and the therapeutic groups at the same period respectively; the expression of α-SMA+ positive cells had the same tendency as CTGF mRNA and protein. [Conclusion] The hepatic expression of CTGF is correlated to the murine schistosomiasis liver fibrosis; CTGF may take its biological effects by its action on HSCs.