CAJ | 학술논문

目的观察鸡羽根角蛋白管作为组织工程材料的毒性作用.方法分别制备不同处理强度的3种鸡羽根角蛋白管的浸提液并测定其化学成分.采用皮肤致敏实验(大鼠)、皮内刺激实验(家兔)、小鼠全身急性毒性实验以及体外细胞毒性实验观察鸡羽根角蛋白管浸提液的体内和体外毒性作用.结果鸡羽根在浸出液中释放的主要物质为中等分子量的肽类物质,大分子蛋白释放不多.皮肤致敏实验和皮内刺激实验显示对浸提液不引起皮肤及皮内红斑、水肿和坏死现象.小鼠全身急性毒性实验中,腹腔注射浸提液的小鼠未出现死亡、衰竭、发绀、震颤、严重的腹膜刺激、眼睑下垂及呼吸困难等毒性症状.体外细胞毒性实验示浸提液细胞毒性接近于0级.结论鸡羽根角蛋白管可被水解,释放一些肽类物质,其浸提液对皮肤无致敏作用,皮内刺激反应弱,不引起全身急性毒性症状,无细胞毒性.
목적 관찰계우근각단백관작위조직공정재료적독성작용.방법 분별제비불동처리강도적3충계우근각단백관적침제액병측정기화학성분.채용피부치민실험(대서)、피내자격실험(가토)、소서전신급성독성실험이급체외세포독성실험관찰계우근각단백관침제액적체내화체외독성작용.결과 계우근재침출액중석방적주요물질위중등분자량적태류물질,대분자단백석방불다.피부치민실험화피내자격실험현시대침제액불인기피부급피내홍반、수종화배사현상.소서전신급성독성실험중,복강주사침제액적소서미출현사망、쇠갈、발감、진전、엄중적복막자격、안검하수급호흡곤난등독성증상.체외세포독성실험시침제액세포독성접근우0급.결론 계우근각단백관가피수해,석방일사태류물질,기침제액대피부무치민작용,피내자격반응약,불인기전신급성독성증상,무세포독성.
Objective To evaluate the toxicity of chicken calamus keratin (CCK) conduit as a tissue-engineered scaffold material. Methods The chemical composition of the leaching solution of CCK was determined by means of ultraviolet spectrometry, and the toxic effects of the solution was evaluated by skin sensitization test in rats, intracutaneous stimulation test in rabbits, acute systemic toxicity test in mice, and cytotoxicity test in L929 cells. Results The leaching solution of CCK consisted mainly of middle-molecular-weight peptides with a small quantity of macromolecular proteins. Skin sensitization test in rats showed that application of the CCK leaching solution caused no obvious skin reddening, regional edema, or skin necrosis. Intracutaneous injection of the leaching solution in rabbits did not induce obvious skin stimulation manifested by intradermal erythema or edema. In acute systemic toxic test, administration of the leaching solution in mice caused no death,organ dysfunction, cyanosis, tremor, severe peritoneal irritation, ptosis, or dyspnoea. In ivtro cytotoxicity test indicated that the cell toxicity of the CCK leaching solution was approximately at 0 level. Conclusion CCK contained in the treated chicken calamus easily undergoes hydrolysis to release mainly some peptides which do not induce obvious toxic effects, suggesting the safe potential applications of CCK conduit as a tissue-engineering biometerial.