CAJ | 학술논문

目的建立血糖钳方法,检验所建高脂、高果糖饮食诱发的胰岛素抵抗模型以及谷氨酸钠(monosodiumglutamate,MSG)所致的肥胖性大鼠胰岛素抵抗模型.方法腹腔注射小剂量链脲霉素(streptozotocin,STZ),30mg/kg轻度损伤胰岛β细胞,造成大鼠糖耐量异常后加喂高果糖高脂肪饲料,并饮用10%果糖水,造成胰岛素抵抗.吡格列酮20mg/kg@d-1治疗后,以血糖钳方法测定葡萄糖输注速率(glucose infusion rate,GIR);另一模型为具胰岛素抵抗特征的MSG肥胖性大鼠.新生Wistar大鼠出生第二天起皮下注射L-谷氨酸钠4g/kg@d 1,连续7天,造成肥胖.10月龄时,用中药复方千金黄连丸加味提取物,相当于生药10g/kg@d-1治疗,并以血糖钳方法测定对葡萄糖输注速率的影响,且分析了反映胰岛素敏感性的GIR值同血脂、脂肪重、体重、血胰岛素水平及胰岛素敏感指数等指标的相关性.结果两模型组同相应正常对照组相比,胰岛素敏感性均明显降低,给药后均有显著提高.且GIR反映的敏感性同胰岛素敏感指数呈较好的正相关,而与空腹血胰岛素水平、血甘油三酯、血总胆固醇以及腹脂重、腹脂/体重值呈负相关.结论高脂高果糖饮食诱发的大鼠模型及MSG肥胖性大鼠均具有明显的胰岛素抵抗,分别以吡格列酮以及中药复方提取物治疗后,抵抗性均有明显改善.
목적건립혈당겸방법,검험소건고지、고과당음식유발적이도소저항모형이급곡안산납(monosodiumglutamate,MSG)소치적비반성대서이도소저항모형.방법복강주사소제량련뇨매소(streptozotocin,STZ),30mg/kg경도손상이도β세포,조성대서당내량이상후가위고과당고지방사료,병음용10%과당수,조성이도소저항.필격렬동20mg/kg@d-1치료후,이혈당겸방법측정포도당수주속솔(glucose infusion rate,GIR);령일모형위구이도소저항특정적MSG비반성대서.신생Wistar대서출생제이천기피하주사L-곡안산납4g/kg@d 1,련속7천,조성비반.10월령시,용중약복방천금황련환가미제취물,상당우생약10g/kg@d-1치료,병이혈당겸방법측정대포도당수주속솔적영향,차분석료반영이도소민감성적GIR치동혈지、지방중、체중、혈이도소수평급이도소민감지수등지표적상관성.결과량모형조동상응정상대조조상비,이도소민감성균명현강저,급약후균유현저제고.차GIR반영적민감성동이도소민감지수정교호적정상관,이여공복혈이도소수평、혈감유삼지、혈총담고순이급복지중、복지/체중치정부상관.결론고지고과당음식유발적대서모형급MSG비반성대서균구유명현적이도소저항,분별이필격렬동이급중약복방제취물치료후,저항성균유명현개선.
Objective To set up the method of euglycemic clamp and evaluate insulin resistance having developed in high fat high fructose induced rats and monosodium glutamate (MSG) induced obese rats.Methods Female Wistar rats were injected a low dose of streptozotocin (30mg/kg) intraperitoneally followed by a high fat high fructose diet and drank 10% fructose water. After feding for about 6 weeks, pioglitazone, an insulin sensitizer, was administered at the dose of 20mg/kg/day and insulin sensitivity was measured with euglycemic clamp technique. The second model was MSG obese rats. Neonatal rats were treated with monosodium glutamate, when 10 months old, the extract of Traditional Chinese Medicine recipe qianjinhuanglianwanjiawei (equal to raw medicinal plant 10g/kg/day) were given for one month, then insulin sensitivity was estimated by euglycemic clamp method in all groups. Moreover, the correlation between glucose infusion rate (GIR) and serum lipid level, adipose tissue weight, body weight, the ratio of adipose weight and body weight, serum insulin level, and insulin sensitivity index (ISI) were respectively analyzed.Results GIR was significantly decreased in the two model rats and improved to the normal level after agents treatment. GIR was well positively correlated with ISI, suggesting they both well reflect the insulin sensitivity.Conclusion It was proved by euglycemic clamp technique that the two models both have the characteristics of significant insulin resistance. Pioglitazone and the extract of the TCM recipe can enhance insulin sensitivity on the two models respectively.