CAJ | 학술논문

利用PCR方法分别构建了烟草花叶病毒(TMV)中一个推测为复制酶的54-kD蛋白基因(54K)缺失N端、C端和仅余基因中部261bp的3个缺失突变体，与野生型54K一起克隆入植物中间载体p208，并通过根癌土壤杆菌(Agrobacteriumtumefaciens (Smith et Townsend) Conn)介导的方法转化烟草(Nicotiana tabacum L.)。用TMV侵染转基因植物的R0代和R1代，结果显示这3个缺失突变体均能介导对TMV的抗病性。
이용PCR방법분별구건료연초화협병독(TMV)중일개추측위복제매적54-kD단백기인(54K)결실N단、C단화부여기인중부261bp적3개결실돌변체，여야생형54K일기극륭입식물중간재체p208，병통과근암토양간균(Agrobacteriumtumefaciens (Smith et Townsend) Conn)개도적방법전화연초(Nicotiana tabacum L.)。용TMV침염전기인식물적R0대화R1대，결과현시저3개결실돌변체균능개도대TMV적항병성。
Three deletion mutants of tobacco mosaic virus (TMV) 54-kDputative replicase gene (54K) were obtained by PCR, and cloned into plant expression vector p208, then transformed into Nicotiana tabacum L. cv. SR1 by Agrobacterium tumefaciens (Smith et Townsend) Conn Ti plasmid-mediated transformation. All the transgenic plants with the N-terminal deletion mutant, the C-terminal deletion mutant and the only 261 nucleotides region from the central part of the 54K ORF showed significant resistance against TMV.