CAJ | 학술논문

目的:了解透析器复用对补体和单个核细胞激活的影响。方法:比较透析器第四次复用与首次使用时，血浆补体终末复合物(SC5b-9) 浓度及分离的单个核细胞(PBMC)在体外培养产生IL-1β、TNF-α和IL-6量的差异。结果:血仿膜透析器用过氧乙酸和福尔马林消毒后第四次复用时，血浆SC5b-9浓度变化幅度较首次使用明显降低；PBMC在LPS刺激下体外培养产生IL-1β、TNF-α和IL-6的量与首次使用相比差异无显著性。结论:血仿膜透析器用过氧乙酸和福尔马林消毒复用，可在一定程度上降低其补体激活能力，但不影响PBMC产生细胞因子。
목적:료해투석기복용대보체화단개핵세포격활적영향。방법:비교투석기제사차복용여수차사용시，혈장보체종말복합물(SC5b-9) 농도급분리적단개핵세포(PBMC)재체외배양산생IL-1β、TNF-α화IL-6량적차이。결과:혈방막투석기용과양을산화복이마림소독후제사차복용시，혈장SC5b-9농도변화폭도교수차사용명현강저；PBMC재LPS자격하체외배양산생IL-1β、TNF-α화IL-6적량여수차사용상비차이무현저성。결론:혈방막투석기용과양을산화복이마림소독복용，가재일정정도상강저기보체격활능력，단불영향PBMC산생세포인자。
Objective：To investig ate the effects of reused hemodialysis membrane and detoxification agents on act ivation of complement and peripheral blood mononuclear cell(PBMC). Methods：The plasma concentration of SC5b-9 complex were measur e d with ELISA, and cytokine production by the cultured PBMC was measured by RIA a t the time before and after the beginning of single session of hemodialysis with a new HE membrane and the fourth reused one detoxificated by Formalin and Perox ide. Results：The plasma concentration of SC5b-9 was remarkably lowe r and compared to a new one,no difference in the cytokine production by PBMC in the patients dialysated by the fou rth reused HE membrane. Conclusions：Reused HE,sterilized with Peroxide or Formal i n, decreases its ability of complement activation, and has no effects on the cyt okine production by PBMC. `